Sensors 2014, 14(7), 13186-13209; doi:10.3390/s140713186

A Large Response Range Reflectometric Urea Biosensor Made from Silica-Gel Nanoparticles

1email, 1,2,* email, 3email, 4email and 2email
Received: 21 December 2013; in revised form: 3 July 2014 / Accepted: 14 July 2014 / Published: 22 July 2014
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A new silica-gel nanospheres (SiO2NPs) composition was formulated, followed by biochemical surface functionalization to examine its potential in urea biosensor development. The SiO2NPs were basically synthesized based on sol–gel chemistry using a modified Stober method. The SiO2NPs surfaces were modified with amine (-NH2) functional groups for urease immobilization in the presence of glutaric acid (GA) cross-linker. The chromoionophore pH-sensitive dye ETH 5294 was physically adsorbed on the functionalized SiO2NPs as pH transducer. The immobilized urease determined urea concentration reflectometrically based on the colour change of the immobilized chromoionophore as a result of the enzymatic hydrolysis of urea. The pH changes on the biosensor due to the catalytic enzyme reaction of immobilized urease were found to correlate with the urea concentrations over a linear response range of 50–500 mM (R2 = 0.96) with a detection limit of 10 mM urea. The biosensor response time was 9 min with reproducibility of less than 10% relative standard deviation (RSD). This optical urea biosensor did not show interferences by Na+, K+, Mg2+ and NH4+ ions. The biosensor performance has been validated using urine samples in comparison with a non-enzymatic method based on the use of p-dimethylaminobenzaldehyde (DMAB) reagent and demonstrated a good correlation between the two different methods (R2 = 0.996 and regression slope of 1.0307). The SiO2NPs-based reflectometric urea biosensor showed improved dynamic linear response range when compared to other nanoparticle-based optical urea biosensors.
Keywords: silica-gel nanospheres; urea biosensor; reflectometric; pH-sensitive; linear response range
PDF Full-text Download PDF Full-Text [961 KB, Updated Version, uploaded 24 July 2014 09:02 CEST]
The original version is still available [1329 KB, uploaded 22 July 2014 12:14 CEST]

Export to BibTeX |

MDPI and ACS Style

Alqasaimeh, M.; Heng, L.Y.; Ahmad, M.; Raj, A.S.; Ling, T.L. A Large Response Range Reflectometric Urea Biosensor Made from Silica-Gel Nanoparticles. Sensors 2014, 14, 13186-13209.

AMA Style

Alqasaimeh M, Heng LY, Ahmad M, Raj AS, Ling TL. A Large Response Range Reflectometric Urea Biosensor Made from Silica-Gel Nanoparticles. Sensors. 2014; 14(7):13186-13209.

Chicago/Turabian Style

Alqasaimeh, Muawia; Heng, Lee Y.; Ahmad, Musa; Raj, A.S. S.; Ling, Tan L. 2014. "A Large Response Range Reflectometric Urea Biosensor Made from Silica-Gel Nanoparticles." Sensors 14, no. 7: 13186-13209.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert