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Sensors 2014, 14(6), 9984-9994; doi:10.3390/s140609984
Article

Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide

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*
University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France
* Author to whom correspondence should be addressed.
Received: 31 March 2014 / Revised: 8 May 2014 / Accepted: 30 May 2014 / Published: 6 June 2014
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in France)
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Abstract

Metalated porphyrins have been described to bind nucleic acids. Additionally, cobalt porphyrins present catalytic properties towards oxygen reduction. In this work, a carboxylic acid-functionalized cobalt porphyrin was physisorbed on reduced graphene oxide, then immobilized on glassy carbon electrodes. The carboxylic groups were used to covalently graft amino-terminated oligonucleotide probes which are complementary to a short microRNA target. It was shown that the catalytic oxygen electroreduction on cobalt porphyrin increases upon hybridization of miRNA strand (“signal-on” response). Current changes are amplified compared to non-catalytic amperometric system. Apart from oxygen, no added reagent is necessary. A limit of detection in the sub-nanomolar range was reached. This approach has never been described in the literature.
Keywords: reduced graphene oxide; microRNA; porphyrin; hybridization; reagentless detection reduced graphene oxide; microRNA; porphyrin; hybridization; reagentless detection
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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De Souza, C.; Zrig, S.; Wang, D.; Pham, M.-C.; Piro, B. Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide. Sensors 2014, 14, 9984-9994.

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