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Sensors 2014, 14(6), 9984-9994; doi:10.3390/s140609984
Article

Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide

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Received: 31 March 2014 / Revised: 8 May 2014 / Accepted: 30 May 2014 / Published: 6 June 2014
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in France)
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Abstract

Metalated porphyrins have been described to bind nucleic acids. Additionally, cobalt porphyrins present catalytic properties towards oxygen reduction. In this work, a carboxylic acid-functionalized cobalt porphyrin was physisorbed on reduced graphene oxide, then immobilized on glassy carbon electrodes. The carboxylic groups were used to covalently graft amino-terminated oligonucleotide probes which are complementary to a short microRNA target. It was shown that the catalytic oxygen electroreduction on cobalt porphyrin increases upon hybridization of miRNA strand (“signal-on” response). Current changes are amplified compared to non-catalytic amperometric system. Apart from oxygen, no added reagent is necessary. A limit of detection in the sub-nanomolar range was reached. This approach has never been described in the literature.
Keywords: reduced graphene oxide; microRNA; porphyrin; hybridization; reagentless detection reduced graphene oxide; microRNA; porphyrin; hybridization; reagentless detection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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De Souza, C.; Zrig, S.; Wang, D.; Pham, M.-C.; Piro, B. Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide. Sensors 2014, 14, 9984-9994.

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