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Sensors 2014, 14(3), 4399-4415; doi:10.3390/s140304399

Electrophoresis-Enhanced Detection of Deoxyribonucleic Acids on a Membrane-Based Lateral Flow Strip Using Avian Influenza H5 Genetic Sequence as the Model

Department of Chemical Engineering, Chung Yuan Christian University, Chung Li, Tao Yuan 32023, Taiwan
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Received: 9 December 2013 / Revised: 14 February 2014 / Accepted: 28 February 2014 / Published: 5 March 2014
(This article belongs to the Special Issue Molecular Sensing and Molecular Electronics)
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Abstract

This study reports a simple strategy to detect a deoxyribonucleic acid (DNA) on a membrane-based lateral flow (MBLF) strip without tedious gel preparation, gel electrophoresis, and EtBr-staining processes. The method also enhances the detection signal of the genetic sample. A direct electric field was applied over two ends of the MBLF strips to induce an electrophoresis of DNAs through the strips. The signal enhancement was demonstrated by the detection of the H5 subtype of avian influenza virus (H5 AIV). This approach showed an excellent selectivity of H5 AIV from other two control species, Arabidopsis thaliana and human PSMA5. It also showed an effective signal repeatability and sensitivity over a series of analyte concentrations. Its detection limit could be enhanced, from 40 ng to 0.1 ng by applying 12 V. The nano-gold particles for the color development were labeled on the capture antibody, and UV-VIS and TEM were used to check if the labeling was successful. This detection strategy could be further developed to apply on the detection of drug-allergic genes at clinics or detection of infectious substances at incident sites by a simple manipulation with an aid of a mini-PCR machine and auxiliary kits.
Keywords: membrane-based lateral-flow detection; DNA detection; electrophoresis; avian influenza; biosensors membrane-based lateral-flow detection; DNA detection; electrophoresis; avian influenza; biosensors
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wu, J.-C.; Chen, C.-H.; Fu, J.-W.; Yang, H.-C. Electrophoresis-Enhanced Detection of Deoxyribonucleic Acids on a Membrane-Based Lateral Flow Strip Using Avian Influenza H5 Genetic Sequence as the Model. Sensors 2014, 14, 4399-4415.

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