Next Article in Journal
A Vibration-Based MEMS Piezoelectric Energy Harvester and Power Conditioning Circuit
Previous Article in Journal
Vertical Dynamic Deflection Measurement in Concrete Beams with the Microsoft Kinect
Article Menu

Export Article

Open AccessArticle
Sensors 2014, 14(2), 3308-3322; doi:10.3390/s140203308

Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy

LNR Campylobacter, IZSAM G.Caporale, via Campo Boario 64100 Teramo, Italy
*
Author to whom correspondence should be addressed.
Received: 22 November 2013 / Revised: 12 February 2014 / Accepted: 14 February 2014 / Published: 19 February 2014
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [720 KB, uploaded 21 June 2014]   |  

Abstract

Campylobacter has developed resistance to several antimicrobial agents over the years, including macrolides, quinolones and fluoroquinolones, becoming a significant public health hazard. A total of 145 strains derived from raw milk, chicken faeces, chicken carcasses, cattle faeces and human faeces collected from various Italian regions, were screened for antimicrobial susceptibility, molecular characterization (SmaI pulsed-field gel electrophoresis) and detection of virulence genes (sequencing and DNA microarray analysis). The prevalence of C. jejuni and C. coli was 62.75% and 37.24% respectively. Antimicrobial susceptibility revealed a high level of resistance for ciprofloxacin (62.76%), tetracycline (55.86%) and nalidixic acid (55.17%). Genotyping of Campylobacter isolates using PFGE revealed a total of 86 unique SmaI patterns. Virulence gene profiles were determined using a new microbial diagnostic microarray composed of 70-mer oligonucleotide probes targeting genes implicated in Campylobacter pathogenicity. Correspondence between PFGE and microarray clusters was observed. Comparisons of PFGE and virulence profiles reflected the high genetic diversity of the strains examined, leading us to speculate different degrees of pathogenicity inside Campylobacter populations.
Keywords: antimicrobial susceptibility; Campylobacter; microarray; PFGE; sequencing; virulence antimicrobial susceptibility; Campylobacter; microarray; PFGE; sequencing; virulence
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Di Giannatale, E.; Di Serafino, G.; Zilli, K.; Alessiani, A.; Sacchini, L.; Garofolo, G.; Aprea, G.; Marotta, F. Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy. Sensors 2014, 14, 3308-3322.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top