Next Article in Journal
A Velocity-Based Impedance Control System for a Low Impact Docking Mechanism (LIDM)
Next Article in Special Issue
Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
Previous Article in Journal
A Split G-Quadruplex and Graphene Oxide-Based Low-Background Platform for Fluorescence Authentication of Pseudostellaria heterophylla
Article Menu

Export Article

Open AccessArticle
Sensors 2014, 14(12), 22982-22997; doi:10.3390/s141222982

Formation of DNA Adducts by Ellipticine and Its Micellar Form in Rats — A Comparative Study

1
Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, CZ-128-40 Prague 2, Czech Republic
2
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613-00 Brno, Czech Republic
3
Division of Preventive Oncology, National Center for Tumor Diseases, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
*
Author to whom correspondence should be addressed.
Received: 28 October 2014 / Revised: 27 November 2014 / Accepted: 28 November 2014 / Published: 3 December 2014
View Full-Text   |   Download PDF [2404 KB, uploaded 3 December 2014]   |  

Abstract

The requirements for early diagnostics as well as effective treatment of cancer diseases have increased the pressure on development of efficient methods for targeted drug delivery as well as imaging of the treatment success. One of the most recent approaches covering the drug delivery aspects is benefitting from the unique properties of nanomaterials. Ellipticine and its derivatives are efficient anticancer compounds that function through multiple mechanisms. Formation of covalent DNA adducts after ellipticine enzymatic activation is one of the most important mechanisms of its pharmacological action. In this study, we investigated whether ellipticine might be released from its micellar (encapsulated) form to generate covalent adducts analogous to those formed by free ellipticine. The 32P-postlabeling technique was used as a useful imaging method to detect and quantify covalent ellipticine-derived DNA adducts. We compared the efficiencies of free ellipticine and its micellar form (the poly(ethylene oxide)-block-poly(allyl glycidyl ether) (PAGE-PEO) block copolymer, P 119 nanoparticles) to form ellipticine-DNA adducts in rats in vivo. Here, we demonstrate for the first time that treatment of rats with ellipticine in micelles resulted in formation of ellipticine-derived DNA adducts in vivo and suggest that a gradual release of ellipticine from its micellar form might produce the enhanced permeation and retention effect of this ellipticine-micellar delivery system. View Full-Text
Keywords: ellipticine; ellipticine-derived DNA adducts; 32P-postlabeling imaging; nanoparticles; micelles ellipticine; ellipticine-derived DNA adducts; 32P-postlabeling imaging; nanoparticles; micelles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Stiborova, M.; Manhartova, Z.; Hodek, P.; Adam, V.; Kizek, R.; Frei, E. Formation of DNA Adducts by Ellipticine and Its Micellar Form in Rats — A Comparative Study. Sensors 2014, 14, 22982-22997.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top