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Sensors 2014, 14(12), 22971-22981; doi:10.3390/s141222971

A Split G-Quadruplex and Graphene Oxide-Based Low-Background Platform for Fluorescence Authentication of Pseudostellaria heterophylla

1
Institute of Drug Research, Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China
2
Fujian Quanzhou Children's Hospital, Quanzhou 362000, China
3
The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
*
Author to whom correspondence should be addressed.
Received: 14 October 2014 / Revised: 9 November 2014 / Accepted: 27 November 2014 / Published: 3 December 2014
(This article belongs to the Section Biosensors)
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Abstract

A label-free split G-quadruplex and graphene oxide (GO)-based fluorescence platform has been designed to distinguish Pseudostellaria heterophylla (PH) from its adulterants based on the differences in their nrDNA ITS sequences. Herein, GO has been first introduced to capture G-rich probes with 2:2 split mode and then decrease the background signal. As T-DNA exists, the probes leave the GO surface to form double-stranded structures followed by the formation of the overhanging G-rich sequence into a G-quadruplex structure, which combines quinaldine red specifically to produce a strong fluorescence signal. In addition, this strategy allows detection of T-DNA in a wide range of concentrations from 1.0 × 10−8 to 2.0 × 10−6 mol·L−1 with a detection limit of 7.8 × 10−9 mol·L−1. We hope that the split G-quadruplex/GO platform can be utilized to further develop gene identification sensors in Traditional Chinese Medicine or other analysis areas. View Full-Text
Keywords: split G-quadruplex; graphene oxide; fluorescence; Pseudostellaria heterophylla split G-quadruplex; graphene oxide; fluorescence; Pseudostellaria heterophylla
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zheng, Z.; Hu, J.; He, Z. A Split G-Quadruplex and Graphene Oxide-Based Low-Background Platform for Fluorescence Authentication of Pseudostellaria heterophylla. Sensors 2014, 14, 22971-22981.

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