Next Article in Journal
Biosignal PI, an Affordable Open-Source ECG and Respiration Measurement System
Next Article in Special Issue
Simultaneous Automatic Electrochemical Detection of Zinc, Cadmium, Copper and Lead Ions in Environmental Samples Using a Thin-Film Mercury Electrode and an Artificial Neural Network
Previous Article in Journal
Analysis of Latency Performance of Bluetooth Low Energy (BLE) Networks
Previous Article in Special Issue
Formation of DNA Adducts by Ellipticine and Its Micellar Form in Rats — A Comparative Study
Article Menu

Export Article

Open AccessArticle
Sensors 2015, 15(1), 79-92; doi:10.3390/s150100079

Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM

1,†
,
1,†
and
1,2,*
1
CEITEC MU, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
2
Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 24 October 2014 / Accepted: 11 December 2014 / Published: 23 December 2014
View Full-Text   |   Download PDF [1471 KB, uploaded 29 December 2014]   |  

Abstract

Label-free immunosensors are well suited for detection of microorganisms because of their fast response and reasonable sensitivity comparable to infection doses of common pathogens. Active (lever oscillator and frequency counter) and passive (impedance analyzer) modes of quartz crystal microbalance (QCM) were used and compared for rapid detection of three strains of E. coli. Different approaches for antibody immobilization were compared, the immobilization of reduced antibody using Sulfo‑SMCC was most effective achieving the limit of detection (LOD) 8 × 104 CFU·mL−1 in 10 min. For the passive mode, software evaluating impedance characteristics in real-time was developed and used. Almost the same results were achieved using both active and passive modes confirming that the sensor properties are not limited by the frequency evaluation method but mainly by affinity of the antibody. Furthermore, reference measurements were done using surface plasmon resonance. Effect of condition of cells on signal was observed showing that cells ruptured by ultrasonication provided slightly higher signal changes than intact microbes. View Full-Text
Keywords: Escherichia coli; immunosensor; impedance analysis; label-free detection; quartz crystal microbalance Escherichia coli; immunosensor; impedance analysis; label-free detection; quartz crystal microbalance
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Farka, Z.; Kovář, D.; Skládal, P. Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors 2015, 15, 79-92.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top