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Sensors 2014, 14(10), 18886-18897; doi:10.3390/s141018886

Heterogeneous Electrochemical Immunoassay of Hippuric Acid on the Electrodeposited Organic Films

1
Department of chemistry, College of Natural Science, Dankook University, Anseo-Dong, Cheonan 330-714, Korea
2
Department of biology, College of Natural Science, Dankook University, Anseo-Dong, Cheonan 330-714, Korea
*
Author to whom correspondence should be addressed.
Received: 24 July 2014 / Revised: 5 September 2014 / Accepted: 25 September 2014 / Published: 13 October 2014
(This article belongs to the Special Issue Immunosensors 2014)
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Abstract

By directly coordinating hippuric acid (HA) to the ferrate (Fe) as an electron transfer mediator, we synthesized a Fe-HA complex, which shows a good electrochemical signal and thus enables the electrochemical immunoanalysis for HA. We electrodeposited organic films containing imidazole groups on the electrode surface and then bonded Ni ion (positive charge) to induce immobilization of Fe-HA (negative charge) through the electrostatic interaction. The heterogeneous competitive immunoassay system relies on the interaction between immobilized Fe-HA antigen conjugate and free HA antigen to its antibody (anti-HA). The electric signal becomes weaker due to the hindered electron transfer reaction when a large-sized HA antibody is bound onto the Fe-HA. However, in the presence of HA, the electric signal increases because free HA competitively reacts with the HA antibody prior to actual reaction and thus prevents the HA antibody from interacting with Fe-HA at the electrode surface. This competition reaction enabled an electrochemical quantitative analysis of HA concentration with a detection limit of 0.5 μg mL−1, and thus allowed us to develop a simple and rapid electrochemical immunosensor. View Full-Text
Keywords: hippuric acid; toluene; electrochemical immunosensor hippuric acid; toluene; electrochemical immunosensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Choi, Y.-B.; Kim, N.-H.; Kim, S.-H.; Tae, G.-S.; Kim, H.-H. Heterogeneous Electrochemical Immunoassay of Hippuric Acid on the Electrodeposited Organic Films. Sensors 2014, 14, 18886-18897.

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