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Sensors 2014, 14(9), 16434-16453; doi:10.3390/s140916434

Love Wave Immunosensor for the Detection of Carbaryl Pesticide

1
Grupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, Spain
2
Sensors, Microsystems and Actuators Laboratory of Louvain (SMALL), ICTEAM Institute, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium
3
Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano (I3BH), Universitat Politècnica de València, 46022 Valencia, Spain
Authors who currently work at AWSensors Corp., Valencia 46023, Spain;
*
Author to whom correspondence should be addressed.
Received: 24 June 2014 / Revised: 11 August 2014 / Accepted: 21 August 2014 / Published: 3 September 2014
(This article belongs to the Special Issue Immunosensors 2014)
View Full-Text   |   Download PDF [2311 KB, uploaded 9 September 2014]   |  

Abstract

A Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14–1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels. View Full-Text
Keywords: biosensor; carbaryl pesticide detection; immunosensor; Love Wave sensor; Surface Acoustic Wave (SAW) biosensor; carbaryl pesticide detection; immunosensor; Love Wave sensor; Surface Acoustic Wave (SAW)
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Rocha-Gaso, M.-I.; García, J.-V.; García, P.; March-Iborra, C.; Jiménez, Y.; Francis, L.-A.; Montoya, Á.; Arnau, A. Love Wave Immunosensor for the Detection of Carbaryl Pesticide. Sensors 2014, 14, 16434-16453.

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