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Sensors 2014, 14(11), 21872-21888; doi:10.3390/s141121872

Rapid Detection of Chloramphenicol Residues in Aquatic Products Using Colloidal Gold Immunochromatographic Assay

1
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
2
Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200000, China
3
Yangtze Delta Region Institute of Tsinghua University, Jiaxing 310013, China
*
Author to whom correspondence should be addressed.
Received: 30 July 2014 / Revised: 15 September 2014 / Accepted: 4 November 2014 / Published: 18 November 2014
(This article belongs to the Special Issue Immunosensors 2014)
View Full-Text   |   Download PDF [2757 KB, uploaded 18 November 2014]   |  

Abstract

A colloidal gold immunochromatographic assay (GICA) was developed for rapid detection of chloramphenicol (CAP) residues in aquatic products. A nitrocellulose (NC) membrane was used as the carrier, and the polyclonal CAP antibody was used as the marker protein. The average diameter of as-prepared colloidal gold nanoparticles (AuNPs) was about 20 nm. The optimal pH value of colloidal gold solutions and the amount of the antibody of CAP were 8.0 and 7.2 μg/mL, respectively. The CAP antibody was immobilized onto the conjugate pad after purification. The CAP conjugate and goat anti-rabbit IgG (secondary antibody) were coated onto the NC membrane. Next, the non-specific sites were blocked with 1% bovine serum albumin. The minimum detectable concentration of CAP in standard solution is 0.5 ng/mL, with good reproducibility. For the real samples from crucian carps injected with a single-dose of CAP in the dorsal muscles, the minimum detectable concentration of CAP residues was 0.5 µg/kg. The chromatographic analysis time was less than 10 min, and the strip had a long storage lifetime of more than 90 days at different temperatures. The strips provide a means for rapid detection of CAP residues in aquatic products. View Full-Text
Keywords: chloramphenicol; colloidal gold nanoparticles; fishery drug residues; immunochromatographic assay chloramphenicol; colloidal gold nanoparticles; fishery drug residues; immunochromatographic assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhou, C.; Zhang, X.; Huang, X.; Guo, X.; Cai, Q.; Zhu, S. Rapid Detection of Chloramphenicol Residues in Aquatic Products Using Colloidal Gold Immunochromatographic Assay. Sensors 2014, 14, 21872-21888.

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