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Sensors 2013, 13(7), 8595-8611; doi:10.3390/s130708595
Article

Direct Electrochemistry of Hemoglobin Immobilized on a Functionalized Multi-Walled Carbon Nanotubes and Gold Nanoparticles Nanocomplex-Modified Glassy Carbon Electrode

1,* , 1
, 1
, 2,* , 2
 and 3
Received: 13 May 2013; in revised form: 17 June 2013 / Accepted: 30 June 2013 / Published: 5 July 2013
(This article belongs to the Special Issue Nanotube and Nanowire Sensors)
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Abstract: Direct electron transfer of hemoglobin (Hb) was realized by immobilizing Hb on a carboxyl functionalized multi-walled carbon nanotubes (FMWCNTs) and gold nanoparticles (AuNPs) nanocomplex-modified glassy carbon electrode. The ultraviolet-visible absorption spectrometry (UV-Vis), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) methods were utilized for additional characterization of the AuNPs and FMWCNTs. The cyclic voltammogram of the modified electrode has a pair of well-defined quasi-reversible redox peaks with a formal potential of −0.270 ± 0.002 V (vs. Ag/AgCl) at a scan rate of 0.05 V/s. The heterogeneous electron transfer constant (ks) was evaluated to be 4.0 ± 0.2 s−1. The average surface concentration of electro-active Hb on the surface of the modified glassy carbon electrode was calculated to be 6.8 ± 0.3 × 10−10 mol cm−2. The cathodic peak current of the modified electrode increased linearly with increasing concentration of hydrogen peroxide (from 0.05 nM to 1 nM) with a detection limit of 0.05 ± 0.01 nM. The apparent Michaelis-Menten constant (Kmapp) was calculated to be 0.85 ± 0.1 nM. Thus, the modified electrode could be applied as a third generation biosensor with high sensitivity, long-term stability and low detection limit.
Keywords: hemoglobin; direct electrochemistry; functionalized multi-walled carbon nanotubes; gold nanoparticles; nanocomplex hemoglobin; direct electrochemistry; functionalized multi-walled carbon nanotubes; gold nanoparticles; nanocomplex
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hong, J.; Zhao, Y.-X.; Xiao, B.-L.; Moosavi-Movahedi, A.A.; Ghourchian, H.; Sheibani, N. Direct Electrochemistry of Hemoglobin Immobilized on a Functionalized Multi-Walled Carbon Nanotubes and Gold Nanoparticles Nanocomplex-Modified Glassy Carbon Electrode. Sensors 2013, 13, 8595-8611.

AMA Style

Hong J, Zhao Y-X, Xiao B-L, Moosavi-Movahedi AA, Ghourchian H, Sheibani N. Direct Electrochemistry of Hemoglobin Immobilized on a Functionalized Multi-Walled Carbon Nanotubes and Gold Nanoparticles Nanocomplex-Modified Glassy Carbon Electrode. Sensors. 2013; 13(7):8595-8611.

Chicago/Turabian Style

Hong, Jun; Zhao, Ying-Xue; Xiao, Bao-Lin; Moosavi-Movahedi, Ali A.; Ghourchian, Hedayatollah; Sheibani, Nader. 2013. "Direct Electrochemistry of Hemoglobin Immobilized on a Functionalized Multi-Walled Carbon Nanotubes and Gold Nanoparticles Nanocomplex-Modified Glassy Carbon Electrode." Sensors 13, no. 7: 8595-8611.


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