Next Article in Journal
Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate
Previous Article in Journal
Optimal Periodic Cooperative Spectrum Sensing Based on Weight Fusion in Cognitive Radio Networks
Article Menu

Export Article

Open AccessArticle
Sensors 2013, 13(4), 5273-5285; https://doi.org/10.3390/s130405273

Development of Robust and Standardized Cantilever Sensors Based on Biotin/Neutravidin Coupling for Antibody Detection

1
Swiss Nano Institute, University of Basel, Klingelbergstrasse 82, 4056 Basel, Switzerland
2
Concentris GmbH, Davidsbodenstrasse 63, 4056 Basel, Switzerland
*
Author to whom correspondence should be addressed.
Received: 14 March 2013 / Revised: 10 April 2013 / Accepted: 15 April 2013 / Published: 19 April 2013
(This article belongs to the Section Biosensors)
Full-Text   |   PDF [956 KB, uploaded 21 June 2014]   |  

Abstract

A cantilever-based protein biosensor has been developed providing a customizable multilayer platform for the detection of antibodies. It consists of a biotin-terminated PEG layer pre-functionalized on the gold-coated cantilever surface, onto which NeutrAvidin is adsorbed through biotin/NeutrAvidin specific binding. NeutrAvidin is used as a bridge layer between the biotin-coated surface and the biotinylated biomolecules, such as biotinylated bovine serum albumin (biotinylated BSA), forming a multilayer sensor for direct antibody capture. The cantilever biosensor has been successfully applied to the detection of mouse anti-BSA (m-IgG) and sheep anti-BSA(s-IgG) antibodies. As expected, the average differential surface stress signals of about 5.7 ± 0.8 ´ 10−3 N/m are very similar for BSA/m-IgG and BSA/s-IgG binding, i.e., they are independent of the origin of the antibody. A statistic evaluation of 112 response curves confirms that the multilayer protein cantilever biosensor shows high reproducibility. As a control test, a biotinylated maltose binding protein was used for detecting specificity of IgG, the result shows a signal of bBSA layer in response to antibody is 5.8 ´ 10−3 N/m compared to bMBP. The pre-functionalized biotin/PEG cantilever surface is found to show a long shelf-life of at least 40 days and retains its responsivity of above 70% of the signal when stored in PBS buffer at 4 °C. The protein cantilever biosensor represents a rapid, label-free, sensitive and reliable detection technique for a real-time protein assay. View Full-Text
Keywords: cantilever; biosensor; protein; multilayer; NeutrAvidin; biotin cantilever; biosensor; protein; multilayer; NeutrAvidin; biotin
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Zhang, J.; Lang, H.P.; Battiston, F.; Backmann, N.; Huber, F.; Gerber, C. Development of Robust and Standardized Cantilever Sensors Based on Biotin/Neutravidin Coupling for Antibody Detection. Sensors 2013, 13, 5273-5285.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top