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Sensors 2013, 13(4), 5273-5285; doi:10.3390/s130405273
Article

Development of Robust and Standardized Cantilever Sensors Based on Biotin/Neutravidin Coupling for Antibody Detection

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Received: 14 March 2013; in revised form: 10 April 2013 / Accepted: 15 April 2013 / Published: 19 April 2013
(This article belongs to the Section Biosensors)
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Abstract: A cantilever-based protein biosensor has been developed providing a customizable multilayer platform for the detection of antibodies. It consists of a biotin-terminated PEG layer pre-functionalized on the gold-coated cantilever surface, onto which NeutrAvidin is adsorbed through biotin/NeutrAvidin specific binding. NeutrAvidin is used as a bridge layer between the biotin-coated surface and the biotinylated biomolecules, such as biotinylated bovine serum albumin (biotinylated BSA), forming a multilayer sensor for direct antibody capture. The cantilever biosensor has been successfully applied to the detection of mouse anti-BSA (m-IgG) and sheep anti-BSA(s-IgG) antibodies. As expected, the average differential surface stress signals of about 5.7 ± 0.8 ´ 10−3 N/m are very similar for BSA/m-IgG and BSA/s-IgG binding, i.e., they are independent of the origin of the antibody. A statistic evaluation of 112 response curves confirms that the multilayer protein cantilever biosensor shows high reproducibility. As a control test, a biotinylated maltose binding protein was used for detecting specificity of IgG, the result shows a signal of bBSA layer in response to antibody is 5.8 ´ 10−3 N/m compared to bMBP. The pre-functionalized biotin/PEG cantilever surface is found to show a long shelf-life of at least 40 days and retains its responsivity of above 70% of the signal when stored in PBS buffer at 4 °C. The protein cantilever biosensor represents a rapid, label-free, sensitive and reliable detection technique for a real-time protein assay.
Keywords: cantilever; biosensor; protein; multilayer; NeutrAvidin; biotin cantilever; biosensor; protein; multilayer; NeutrAvidin; biotin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhang, J.; Lang, H.P.; Battiston, F.; Backmann, N.; Huber, F.; Gerber, C. Development of Robust and Standardized Cantilever Sensors Based on Biotin/Neutravidin Coupling for Antibody Detection. Sensors 2013, 13, 5273-5285.

AMA Style

Zhang J, Lang HP, Battiston F, Backmann N, Huber F, Gerber C. Development of Robust and Standardized Cantilever Sensors Based on Biotin/Neutravidin Coupling for Antibody Detection. Sensors. 2013; 13(4):5273-5285.

Chicago/Turabian Style

Zhang, Jiayun; Lang, Hans P.; Battiston, Felice; Backmann, Natalija; Huber, Francois; Gerber, Christoph. 2013. "Development of Robust and Standardized Cantilever Sensors Based on Biotin/Neutravidin Coupling for Antibody Detection." Sensors 13, no. 4: 5273-5285.


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