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Caffeine as a Potential Quorum Sensing Inhibitor
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 25 February 2013; in revised form: 10 April 2013 / Accepted: 16 April 2013 / Published: 18 April 2013
Abstract: Quorum sensing enables bacteria to control the gene expression in response to the cell density. It regulates a variety of bacterial physiological functions such as biofilm formation, bioluminescence, virulence factors and swarming which has been shown contribute to bacterial pathogenesis. The use of quorum sensing inhibitor would be of particular interest in treating bacterial pathogenicity and infections. In this work, we have tested caffeine as quorum sensing inhibitor by using Chromobacterium violaceum CV026 as a biosensor. We verified that caffeine did not degrade the N-acyl homoserine lactones tested. In this work, it is shown that caffeine could inhibit N-acyl homoserine lactone production and swarming of a human opportunistic pathogen, namely Pseudomonas aeruginosa PA01. To the best of our knowledge, this is the first documentation providing evidence on the presence of anti-quorum sensing activity in caffeine. Our work will allow caffeine to be explored as anti-infective drugs.
Keywords: AHL synthesis inhibition; anti-infective drugs; Chromobacterium violaceum CV026; N-acyl-L-homoserine lactones (AHL); Pseudomonas aeruginosa PA01; swarming; quorum sensing inhibitor; virulence
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MDPI and ACS Style
Norizan, S.N.M.; Yin, W.-F.; Chan, K.-G. Caffeine as a Potential Quorum Sensing Inhibitor. Sensors 2013, 13, 5117-5129.
Norizan SNM, Yin W-F, Chan K-G. Caffeine as a Potential Quorum Sensing Inhibitor. Sensors. 2013; 13(4):5117-5129.
Norizan, Siti N.M.; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Caffeine as a Potential Quorum Sensing Inhibitor." Sensors 13, no. 4: 5117-5129.