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• Nikolova, B
• Kostadinova, A
• Dimitrov, B
• Zhelev, Z
• Bakalova, R
• Aoki, I
• Saga, T
• Tsoneva, I
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• Zhelev, Z
• Bakalova, R
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• Saga, T
• Tsoneva, I
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Sensors 2013, 13(3), 3625-3634; doi:10.3390/s130303625

Article

Fluorescent Imaging for Assessment of the Effect of Combined Application of Electroporation and Rifampicin on HaCaT Cells as a New Therapeutic Approach for Psoriasis

Biliana Nikolova ^1,† , Anelia Kostadinova ^1,† , Borislav Dimitrov ¹ , Zhivko Zhelev ^1,2 , Rumiana Bakalova ^3,4,* , Ichio Aoki ³ , Tsuneo Saga ³  and Iana Tsoneva ¹

¹ Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria ² Medical Faculty, Trakia University, 11 Armeiska Str., Stara Zagora 6000, Bulgaria ³ Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan ⁴ Medical Faculty, Sofia University, 1 Koziak Str., Sofia 1407, Bulgaria ^† These authors contributed equally to this work.

* Author to whom correspondence should be addressed.

Received: 11 January 2013; in revised form: 13 February 2013 / Accepted: 6 March 2013 / Published: 14 March 2013

(This article belongs to the Special Issue Medical & Biological Imaging)

Download PDF Full-Text [637 KB, uploaded 14 March 2013 11:16 CET]

Abstract: The study aimed to clarify the role of electric pulses in combination with chemotherapy on the viability of keratinocyte cell line HaCaT, in the context of its application as a new therapeutic approach for psoriasis. The data show that electroporation of HaCaT cells in combination with rifampicin induces cytoskeleton disruption and increases permeability of cell monolayer due to cell-cell junctions’ interruption, visualized by fluorescent imaging of E-cadherin and actin integrity. This was accompanied with synergistic reduction of cell viability. The study proposes a new opportunity for more effective skin treatment than chemotherapy. The future application of this electrochemotherapeutic approach for combined local treatment of psoriasis may have serous benefits because of a high possibility to avoid side-effects of conventional chemotherapy.

Keywords: fluorescent imaging; electroporation; electrochemotherapy; rifampicin; psoriasis

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