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Sensors 2013, 13(3), 3625-3634; doi:10.3390/s130303625
Article

Fluorescent Imaging for Assessment of the Effect of Combined Application of Electroporation and Rifampicin on HaCaT Cells as a New Therapeutic Approach for Psoriasis

1,†
,
1,†
,
1
,
1,2
,
3,4,* , 3
,
3
 and
1
1 Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria 2 Medical Faculty, Trakia University, 11 Armeiska Str., Stara Zagora 6000, Bulgaria 3 Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan 4 Medical Faculty, Sofia University, 1 Koziak Str., Sofia 1407, Bulgaria These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 11 January 2013 / Revised: 13 February 2013 / Accepted: 6 March 2013 / Published: 14 March 2013
(This article belongs to the Special Issue Medical & Biological Imaging)
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Abstract

The study aimed to clarify the role of electric pulses in combination with chemotherapy on the viability of keratinocyte cell line HaCaT, in the context of its application as a new therapeutic approach for psoriasis. The data show that electroporation of HaCaT cells in combination with rifampicin induces cytoskeleton disruption and increases permeability of cell monolayer due to cell-cell junctions’ interruption, visualized by fluorescent imaging of E-cadherin and actin integrity. This was accompanied with synergistic reduction of cell viability. The study proposes a new opportunity for more effective skin treatment than chemotherapy. The future application of this electrochemotherapeutic approach for combined local treatment of psoriasis may have serous benefits because of a high possibility to avoid side-effects of conventional chemotherapy.
Keywords: fluorescent imaging; electroporation; electrochemotherapy; rifampicin; psoriasis fluorescent imaging; electroporation; electrochemotherapy; rifampicin; psoriasis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Nikolova, B.; Kostadinova, A.; Dimitrov, B.; Zhelev, Z.; Bakalova, R.; Aoki, I.; Saga, T.; Tsoneva, I. Fluorescent Imaging for Assessment of the Effect of Combined Application of Electroporation and Rifampicin on HaCaT Cells as a New Therapeutic Approach for Psoriasis. Sensors 2013, 13, 3625-3634.

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