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Sensors 2013, 13(3), 3242-3251; doi:10.3390/s130303242
Article

Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers

1,†
,
2
,
3
 and
1,*
1 DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Ørsteds Plads, 2800 Kgs. Lyngby, Denmark 2 DAKO Denmark A/S, Produktionsvej 42, DK-2600 Glostrup, Denmark 3 Bioneer A/S, Kogle Allé 2, 2970 Hørsholm, Denmark Present address: PCMA Rus, Kaluga Region, Russia.
* Author to whom correspondence should be addressed.
Received: 17 January 2013 / Revised: 22 February 2013 / Accepted: 6 March 2013 / Published: 8 March 2013
(This article belongs to the Special Issue Photonic Crystal Sensors)
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Abstract

We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.
Keywords: microstructured polymer optical fiber; fluorescence; antibodies microstructured polymer optical fiber; fluorescence; antibodies
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Emiliyanov, G.; Høiby, P.E.; Pedersen, L.H.; Bang, O. Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers. Sensors 2013, 13, 3242-3251.

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