A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
AbstractA one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
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Frascella, F.; Ricciardi, S.; Rivolo, P.; Moi, V.; Giorgis, F.; Descrovi, E.; Michelotti, F.; Munzert, P.; Danz, N.; Napione, L.; Alvaro, M.; Bussolino, F. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves. Sensors 2013, 13, 2011-2022.
Frascella F, Ricciardi S, Rivolo P, Moi V, Giorgis F, Descrovi E, Michelotti F, Munzert P, Danz N, Napione L, Alvaro M, Bussolino F. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves. Sensors. 2013; 13(2):2011-2022.Chicago/Turabian Style
Frascella, Francesca; Ricciardi, Serena; Rivolo, Paola; Moi, Valeria; Giorgis, Fabrizio; Descrovi, Emiliano; Michelotti, Francesco; Munzert, Peter; Danz, Norbert; Napione, Lucia; Alvaro, Maria; Bussolino, Federico. 2013. "A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves." Sensors 13, no. 2: 2011-2022.