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Sensors 2013, 13(2), 2011-2022; doi:10.3390/s130202011
Article

A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves

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Received: 31 December 2012; in revised form: 29 January 2013 / Accepted: 4 February 2013 / Published: 5 February 2013
(This article belongs to the Special Issue Photonic Crystal Sensors)
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Abstract: A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
Keywords: surface electromagnetic waves; label-free sensing; fluorescence; multilayers; bragg mirrors surface electromagnetic waves; label-free sensing; fluorescence; multilayers; bragg mirrors
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Frascella, F.; Ricciardi, S.; Rivolo, P.; Moi, V.; Giorgis, F.; Descrovi, E.; Michelotti, F.; Munzert, P.; Danz, N.; Napione, L.; Alvaro, M.; Bussolino, F. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves. Sensors 2013, 13, 2011-2022.

AMA Style

Frascella F, Ricciardi S, Rivolo P, Moi V, Giorgis F, Descrovi E, Michelotti F, Munzert P, Danz N, Napione L, Alvaro M, Bussolino F. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves. Sensors. 2013; 13(2):2011-2022.

Chicago/Turabian Style

Frascella, Francesca; Ricciardi, Serena; Rivolo, Paola; Moi, Valeria; Giorgis, Fabrizio; Descrovi, Emiliano; Michelotti, Francesco; Munzert, Peter; Danz, Norbert; Napione, Lucia; Alvaro, Maria; Bussolino, Federico. 2013. "A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves." Sensors 13, no. 2: 2011-2022.



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