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A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, corso Duca degli Abruzzi 24, Torino 10129, Italy
Dipartimento di Scienze di Base e Applicate per l'Ingegneria, SAPIENZA Università di Roma, via A. Scarpa 16, Roma 00161, Italy
Fraunhofer Institute for Applied Optics and Precision Engineering, Albert-Einstein-Str. 7, Jena 07745, Germany
Dipartimento di Scienze Oncologiche, Istituto per la Ricerca e la Cura del Cancro, Università di Torino, Candiolo 10060, Italy
* Author to whom correspondence should be addressed.
Received: 31 December 2012; in revised form: 29 January 2013 / Accepted: 4 February 2013 / Published: 5 February 2013
Abstract: A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
Keywords: surface electromagnetic waves; label-free sensing; fluorescence; multilayers; bragg mirrors
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Frascella, F.; Ricciardi, S.; Rivolo, P.; Moi, V.; Giorgis, F.; Descrovi, E.; Michelotti, F.; Munzert, P.; Danz, N.; Napione, L.; Alvaro, M.; Bussolino, F. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves. Sensors 2013, 13, 2011-2022.
Frascella F, Ricciardi S, Rivolo P, Moi V, Giorgis F, Descrovi E, Michelotti F, Munzert P, Danz N, Napione L, Alvaro M, Bussolino F. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves. Sensors. 2013; 13(2):2011-2022.
Frascella, Francesca; Ricciardi, Serena; Rivolo, Paola; Moi, Valeria; Giorgis, Fabrizio; Descrovi, Emiliano; Michelotti, Francesco; Munzert, Peter; Danz, Norbert; Napione, Lucia; Alvaro, Maria; Bussolino, Federico. 2013. "A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves." Sensors 13, no. 2: 2011-2022.