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Sensors 2013, 13(2), 2011-2022; doi:10.3390/s130202011
Article

A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves

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1 Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, corso Duca degli Abruzzi 24, Torino 10129, Italy 2 Dipartimento di Scienze di Base e Applicate per l'Ingegneria, SAPIENZA Università di Roma, via A. Scarpa 16, Roma 00161, Italy 3 Fraunhofer Institute for Applied Optics and Precision Engineering, Albert-Einstein-Str. 7, Jena 07745, Germany 4 Dipartimento di Scienze Oncologiche, Istituto per la Ricerca e la Cura del Cancro, Università di Torino, Candiolo 10060, Italy
* Author to whom correspondence should be addressed.
Received: 31 December 2012 / Revised: 29 January 2013 / Accepted: 4 February 2013 / Published: 5 February 2013
(This article belongs to the Special Issue Photonic Crystal Sensors)
View Full-Text   |   Download PDF [441 KB, 21 June 2014; original version 21 June 2014]   |   Browse Figures

Abstract

A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
Keywords: surface electromagnetic waves; label-free sensing; fluorescence; multilayers; bragg mirrors surface electromagnetic waves; label-free sensing; fluorescence; multilayers; bragg mirrors
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Frascella, F.; Ricciardi, S.; Rivolo, P.; Moi, V.; Giorgis, F.; Descrovi, E.; Michelotti, F.; Munzert, P.; Danz, N.; Napione, L.; Alvaro, M.; Bussolino, F. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves. Sensors 2013, 13, 2011-2022.

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