Sensors 2013, 13(10), 13892-13902; doi:10.3390/s131013892
Article

Multi-Channel Hyperspectral Fluorescence Detection Excited by Coupled Plasmon-Waveguide Resonance

1,2email, 3email, 4email, 1email, 1email, 1,2email and 1,* email
1 Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China 2 Department of Physics, Tsinghua University, Beijing 100084, China 3 Laboratory of Advanced Power Source, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China 4 Graduate School at Shenzhen, Harbin Institute of Technology, Shenzhen 518055, China
* Author to whom correspondence should be addressed.
Received: 5 August 2013; in revised form: 6 October 2013 / Accepted: 8 October 2013 / Published: 14 October 2013
(This article belongs to the Section Biosensors)
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Abstract: We propose in this paper a biosensor scheme based on coupled plasmon-waveguide resonance (CPWR) excited fluorescence spectroscopy. A symmetrical structure that offers higher surface electric field strengths, longer surface propagation lengths and depths is developed to support guided waveguide modes for the efficient excitation of fluorescence. The optimal parameters for the sensor films are theoretically and experimentally investigated, leading to a detection limit of 0.1 nM (for a Cy5 solution). Multiplex analysis possible with the fluorescence detection is further advanced by employing the hyperspectral fluorescence technique to record the full spectra for every pixel on the sample plane. We demonstrate experimentally that highly overlapping fluorescence (Cy5 and Dylight680) can be distinguished and ratios of different emission sources can be determined accurately. This biosensor shows great potential for multiplex detections of fluorescence analytes.
Keywords: CPWR excited fluorescence; hyperspectral fluorescence analysis; multi-channel

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MDPI and ACS Style

Du, C.; Liu, L.; Zhang, L.; Guo, J.; Guo, J.; Ma, H.; He, Y. Multi-Channel Hyperspectral Fluorescence Detection Excited by Coupled Plasmon-Waveguide Resonance. Sensors 2013, 13, 13892-13902.

AMA Style

Du C, Liu L, Zhang L, Guo J, Guo J, Ma H, He Y. Multi-Channel Hyperspectral Fluorescence Detection Excited by Coupled Plasmon-Waveguide Resonance. Sensors. 2013; 13(10):13892-13902.

Chicago/Turabian Style

Du, Chan; Liu, Le; Zhang, Lin; Guo, Jun; Guo, Jihua; Ma, Hui; He, Yonghong. 2013. "Multi-Channel Hyperspectral Fluorescence Detection Excited by Coupled Plasmon-Waveguide Resonance." Sensors 13, no. 10: 13892-13902.

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