Sensors 2013, 13(10), 13708-13716; doi:10.3390/s131013708
Article

A Silicon Nanomembrane Detector for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) of Large Proteins

1 Department of Electrical Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 702-701, Korea 2 Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA 3 Center for Hybrid Nanostructures (CHYN), Institute of Applied Physics, University of Hamburg, Jungiussstr. 11, Hamburg 20355, Germany
* Author to whom correspondence should be addressed.
Received: 8 August 2013; in revised form: 22 September 2013 / Accepted: 8 October 2013 / Published: 11 October 2013
(This article belongs to the Special Issue Nanomechanical Sensors)
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Abstract: We describe a MALDI-TOF ion detector based on freestanding silicon nanomembrane technology. The detector is tested in a commercial MALDI-TOF mass spectrometer with equimolar mixtures of proteins. The operating principle of the nanomembrane detector is based on phonon-assisted field emission from these silicon nanomembranes, in which impinging ion packets excite electrons in the nanomembrane to higher energy states. Thereby the electrons can overcome the vacuum barrier and escape from the surface of the nanomembrane via field emission. Ion detection is demonstrated of apomyoglobin (16,952 Da), aldolase (39,212 Da), bovine serum albumin (66,430 Da), and their equimolar mixtures. In addition to the three intact ions, a large number of fragment ions are also revealed by the silicon nanomembrane detector, which are not observable with conventional detectors.
Keywords: silicon nanomembrane; MALDI TOF; mass spectrometry; phonon; ion detector; NEMS

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MDPI and ACS Style

Park, J.; Blick, R.H. A Silicon Nanomembrane Detector for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) of Large Proteins. Sensors 2013, 13, 13708-13716.

AMA Style

Park J, Blick RH. A Silicon Nanomembrane Detector for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) of Large Proteins. Sensors. 2013; 13(10):13708-13716.

Chicago/Turabian Style

Park, Jonghoo; Blick, Robert H. 2013. "A Silicon Nanomembrane Detector for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) of Large Proteins." Sensors 13, no. 10: 13708-13716.

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