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Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore
* Author to whom correspondence should be addressed.
Received: 6 August 2013; in revised form: 9 September 2013 / Accepted: 22 September 2013 / Published: 30 September 2013
Abstract: In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N–hexanoylhomoserine lactone (C6-HSL) and N–octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
Keywords: CepI/CepR homologs; MALDI-TOF mass spectrometry; N-hexanoylhomoserine lactone; tropical soil bacteria; triple quadrupole LC/MS
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MDPI and ACS Style
Chen, J.W.; Koh, C.-L.; Sam, C.-K.; Yin, W.-F.; Chan, K.-G. Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9. Sensors 2013, 13, 13217-13227.
Chen JW, Koh C-L, Sam C-K, Yin W-F, Chan K-G. Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9. Sensors. 2013; 13(10):13217-13227.
Chen, Jian W.; Koh, Chong-Lek; Sam, Choon-Kook; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9." Sensors 13, no. 10: 13217-13227.