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Sensors 2013, 13(10), 13217-13227; doi:10.3390/s131013217
Article

Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9

1
,
2
,
2
,
1
 and
1,*
1 Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia 2 Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore
* Author to whom correspondence should be addressed.
Received: 6 August 2013 / Revised: 9 September 2013 / Accepted: 22 September 2013 / Published: 30 September 2013
(This article belongs to the Section Biosensors)
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Abstract

In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N–hexanoylhomoserine lactone (C6-HSL) and N–octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
Keywords: CepI/CepR homologs; MALDI-TOF mass spectrometry; N-hexanoylhomoserine lactone; tropical soil bacteria; triple quadrupole LC/MS CepI/CepR homologs; MALDI-TOF mass spectrometry; N-hexanoylhomoserine lactone; tropical soil bacteria; triple quadrupole LC/MS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Chen, J.W.; Koh, C.-L.; Sam, C.-K.; Yin, W.-F.; Chan, K.-G. Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9. Sensors 2013, 13, 13217-13227.

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