Sensors 2013, 13(10), 13217-13227; doi:10.3390/s131013217
Article

Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9

1email, 2email, 2email, 1email and 1,* email
Received: 6 August 2013; in revised form: 9 September 2013 / Accepted: 22 September 2013 / Published: 30 September 2013
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N–hexanoylhomoserine lactone (C6-HSL) and N–octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
Keywords: CepI/CepR homologs; MALDI-TOF mass spectrometry; N-hexanoylhomoserine lactone; tropical soil bacteria; triple quadrupole LC/MS
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MDPI and ACS Style

Chen, J.W.; Koh, C.-L.; Sam, C.-K.; Yin, W.-F.; Chan, K.-G. Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9. Sensors 2013, 13, 13217-13227.

AMA Style

Chen JW, Koh C-L, Sam C-K, Yin W-F, Chan K-G. Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9. Sensors. 2013; 13(10):13217-13227.

Chicago/Turabian Style

Chen, Jian W.; Koh, Chong-Lek; Sam, Choon-Kook; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9." Sensors 13, no. 10: 13217-13227.

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