Sensors 2013, 13(10), 12943-12957; doi:10.3390/s131012943
Article

Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters

1email, 2email, 2email, 1email, 1email, 1email and 1,* email
Received: 15 August 2013; in revised form: 8 September 2013 / Accepted: 12 September 2013 / Published: 25 September 2013
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Proteobacteria produce N-acylhomoserine lactones as signaling molecules, which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported work on the degradation of such molecules by basidiomycetous yeast. By using a minimal growth medium containing N-3-oxohexanoylhomoserine lactone as the sole source of carbon, a wetland water sample from Malaysia was enriched for microbial strains that can degrade N-acylhomoserine lactones, and consequently, a basidiomycetous yeast strain WW1C was isolated. Morphological phenotype and molecular analyses confirmed that WW1C was a strain of Trichosporon loubieri. We showed that WW1C degraded AHLs with N-acyl side chains ranging from 4 to 10 carbons in length, with or without oxo group substitutions at the C3 position. Re-lactonisation bioassays revealed that WW1C degraded AHLs via a lactonase activity. To the best of our knowledge, this is the first report of degradation of N-acyl-homoserine lactones and utilization of N-3-oxohexanoylhomoserine as carbon and nitrogen source for growth by basidiomycetous yeast from tropical wetland water; and the degradation of bacterial quorum sensing molecules by an eukaryotic yeast.
Keywords: basidiomycetous; biosensor; lactonase; N-acylhomoserine lactone; quorum sensing; quorum quenching; Rapid Resolution Liquid Chromatography; Trichosporon loubieri; yeast
PDF Full-text Download PDF Full-Text [718 KB, uploaded 21 June 2014 09:20 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Wong, C.-S.; Koh, C.-L.; Sam, C.-K.; Chen, J.W.; Chong, Y.M.; Yin, W.-F.; Chan, K.-G. Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters. Sensors 2013, 13, 12943-12957.

AMA Style

Wong C-S, Koh C-L, Sam C-K, Chen JW, Chong YM, Yin W-F, Chan K-G. Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters. Sensors. 2013; 13(10):12943-12957.

Chicago/Turabian Style

Wong, Cheng-Siang; Koh, Chong-Lek; Sam, Choon-Kook; Chen, Jian W.; Chong, Yee M.; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters." Sensors 13, no. 10: 12943-12957.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert