Abstract: Proteobacteria produce N-acylhomoserine lactones as signaling molecules, which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported work on the degradation of such molecules by basidiomycetous yeast. By using a minimal growth medium containing N-3-oxohexanoylhomoserine lactone as the sole source of carbon, a wetland water sample from Malaysia was enriched for microbial strains that can degrade N-acylhomoserine lactones, and consequently, a basidiomycetous yeast strain WW1C was isolated. Morphological phenotype and molecular analyses confirmed that WW1C was a strain of Trichosporon loubieri. We showed that WW1C degraded AHLs with N-acyl side chains ranging from 4 to 10 carbons in length, with or without oxo group substitutions at the C3 position. Re-lactonisation bioassays revealed that WW1C degraded AHLs via a lactonase activity. To the best of our knowledge, this is the first report of degradation of N-acyl-homoserine lactones and utilization of N-3-oxohexanoylhomoserine as carbon and nitrogen source for growth by basidiomycetous yeast from tropical wetland water; and the degradation of bacterial quorum sensing molecules by an eukaryotic yeast.
Keywords: basidiomycetous; biosensor; lactonase; N-acylhomoserine lactone; quorum sensing; quorum quenching; Rapid Resolution Liquid Chromatography; Trichosporon loubieri; yeast
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Wong, C.-S.; Koh, C.-L.; Sam, C.-K.; Chen, J.W.; Chong, Y.M.; Yin, W.-F.; Chan, K.-G. Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters. Sensors 2013, 13, 12943-12957.
Wong C-S, Koh C-L, Sam C-K, Chen JW, Chong YM, Yin W-F, Chan K-G. Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters. Sensors. 2013; 13(10):12943-12957.
Wong, Cheng-Siang; Koh, Chong-Lek; Sam, Choon-Kook; Chen, Jian W.; Chong, Yee M.; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters." Sensors 13, no. 10: 12943-12957.