Sensors 2013, 13(10), 12794-12803; doi:10.3390/s131012794
Article

A Gold Nanoparticles Enhanced Surface Plasmon Resonance Immunosensor for Highly Sensitive Detection of Ischemia-Modified Albumin

1 Laboratory of Biomedical Engineering, Chongqing Medical University, 1 Yixueyuan Road, Chongqing 400016, China 2 Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, 174 Shapingba Main Street, Chongqing 400044, China
* Authors to whom correspondence should be addressed.
Received: 18 July 2013; in revised form: 12 September 2013 / Accepted: 17 September 2013 / Published: 25 September 2013
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Abstract: In this study a novel sensitive nanogold particle sensor enhancement based on mixed self-assembled monolayers was explored and used to construct a Surface Plasmon Resonance (SPR) immunosensor to detect Ischemia Modified Albumin (IMA). Compared with a direct binding SPR assay at a limit of detection (LOD) of 100 ng/L, gold nanoparticles (AuNPs) of 10 nm dramatically improved the LOD of IMA to 10 ng/L. Meanwhile, no interfering substance that may lead to false positive results was identified. These results suggested that the SPR biosensor presented superior properties, and provided a simple label-free strategy to increase assay sensitivity for further acute coronary syndrome (ACS) diagnosis.
Keywords: ischemia modified albumin; surface plasmon resonance immunosensor; gold nanoparticles; signal enhancement

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MDPI and ACS Style

Li, G.; Li, X.; Yang, M.; Chen, M.-M.; Chen, L.-C.; Xiong, X.-L. A Gold Nanoparticles Enhanced Surface Plasmon Resonance Immunosensor for Highly Sensitive Detection of Ischemia-Modified Albumin. Sensors 2013, 13, 12794-12803.

AMA Style

Li G, Li X, Yang M, Chen M-M, Chen L-C, Xiong X-L. A Gold Nanoparticles Enhanced Surface Plasmon Resonance Immunosensor for Highly Sensitive Detection of Ischemia-Modified Albumin. Sensors. 2013; 13(10):12794-12803.

Chicago/Turabian Style

Li, Guang; Li, Xian; Yang, Meng; Chen, Meng-Meng; Chen, Long-Cong; Xiong, Xing-Liang. 2013. "A Gold Nanoparticles Enhanced Surface Plasmon Resonance Immunosensor for Highly Sensitive Detection of Ischemia-Modified Albumin." Sensors 13, no. 10: 12794-12803.

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