Next Article in Journal
Cognitive LF-Ant: A Novel Protocol for Healthcare Wireless Sensor Networks
Previous Article in Journal
In-Line Fiber Optic Interferometric Sensors in Single-Mode Fibers
Article Menu

Export Article

Open AccessArticle
Sensors 2012, 12(8), 10450-10462; doi:10.3390/s120810450

Mechanisms for the Direct Electron Transfer of Cytochrome c Induced by Multi-Walled Carbon Nanotubes

1
Department of Environmental Engineering, Peking University, Beijing 100871, China
2
The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China
3
School of Food and Bioengineering, Shandong Institute of Light Industry, Jinan 250353, China
*
Author to whom correspondence should be addressed.
Received: 18 June 2012 / Revised: 17 July 2012 / Accepted: 17 July 2012 / Published: 2 August 2012
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [336 KB, uploaded 21 June 2014]   |  

Abstract

Multi-walled carbon nanotube (MWCNT)-modified electrodes can promote the direct electron transfer (DET) of cytochrome c (Cyt c). There are several possible mechanisms that explain the DET of Cyt c. In this study, several experimental methods, including Fourier transform infrared spectroscopy, circular dichroism, ultraviolet-visible absorption spectroscopy, and electron paramagnetic resonance spectroscopy were utilized to investigate the conformational changes of Cyt c induced by MWCNTs. The DET mechanism was demonstrated at various nano-levels: secondary structure, spatial orientation, and spin state. In the presence of MWCNTs, the secondary structure of Cyt c changes, which exposes the active site, then, the orientation of the heme is optimized, revolving the exposed active center to the optimum spatial orientation for DET; and finally, a transition of spin states is induced, providing relatively high energy and a more open microenvironment for electron transfer. These changes at different nano-levels are closely connected and form a complex process that promotes the electron transfer of Cyt c. View Full-Text
Keywords: direct electron transfer (DET); cytochrome c (Cyt c); multi-walled carbon nanotube (MWCNT); secondary structure direct electron transfer (DET); cytochrome c (Cyt c); multi-walled carbon nanotube (MWCNT); secondary structure
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhao, H.-Z.; Du, Q.; Li, Z.-S.; Yang, Q.-Z. Mechanisms for the Direct Electron Transfer of Cytochrome c Induced by Multi-Walled Carbon Nanotubes. Sensors 2012, 12, 10450-10462.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top