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Sensors 2012, 12(6), 7095-7108; doi:10.3390/s120607095
Article

Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples

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NTT Microsystem Integration Laboratories, Atsugi 240198, Japan
* Author to whom correspondence should be addressed.
Received: 15 April 2012 / Revised: 21 May 2012 / Accepted: 22 May 2012 / Published: 29 May 2012
(This article belongs to the Section Biosensors)
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Abstract

We have successfully developed a surface plasmon resonance (SPR) measurement system for the on-site immunoassay of real samples. The system is composed of a portable SPR instrument (290 mm(W) x 160 mm(D) x 120 mm(H)) and a microfluidic immunoassay chip (16 mm(W) x 16 mm(D) x 4 mm(H)) that needs no external pump system. An integrated vertical capillary tube functions as a large volume (150 μL ) passive pump and a waste reservoir that has sufficient capacity for several refill operations. An immunoassay was carried out that employed the direct injection of a buffer and a test sample in sequence into a microfluidic chip that included 9 antibody bands and 10 reference reagent bands immobilized in the flow channel. By subtracting a reliable averaged reference sensorgram from the antibody, we effectively reduced the influence of the non-specific binding, and then our chip successfully detected the specific binding of spiked IgG in non-homogeneous milk. IgG is a model antigen that is certain not to be present in non-homogeneous milk, and non-homogeneous milk is a model of real sample that includes many interfering foreign substances that induce non-specific binding. The direct injection of a real sample with no pretreatment enabled us to complete the entire immunoassay in several minutes. This ease of operation and short measuring time are acceptable for on-site agricultural, environmentaland medical testing.
Keywords: SPR; immunoassay; raw sample; on-site; passive pump; microfluidics SPR; immunoassay; raw sample; on-site; passive pump; microfluidics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Horiuchi, T.; Miura, T.; Iwasaki, Y.; Seyama, M.; Inoue, S.; Takahashi, J.-I.; Haga, T.; Tamechika, E. Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples. Sensors 2012, 12, 7095-7108.

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