Sensors 2012, 12(6), 7095-7108; doi:10.3390/s120607095
Article

Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples

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Received: 15 April 2012; in revised form: 21 May 2012 / Accepted: 22 May 2012 / Published: 29 May 2012
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We have successfully developed a surface plasmon resonance (SPR) measurement system for the on-site immunoassay of real samples. The system is composed of a portable SPR instrument (290 mm(W) x 160 mm(D) x 120 mm(H)) and a microfluidic immunoassay chip (16 mm(W) x 16 mm(D) x 4 mm(H)) that needs no external pump system. An integrated vertical capillary tube functions as a large volume (150 μL ) passive pump and a waste reservoir that has sufficient capacity for several refill operations. An immunoassay was carried out that employed the direct injection of a buffer and a test sample in sequence into a microfluidic chip that included 9 antibody bands and 10 reference reagent bands immobilized in the flow channel. By subtracting a reliable averaged reference sensorgram from the antibody, we effectively reduced the influence of the non-specific binding, and then our chip successfully detected the specific binding of spiked IgG in non-homogeneous milk. IgG is a model antigen that is certain not to be present in non-homogeneous milk, and non-homogeneous milk is a model of real sample that includes many interfering foreign substances that induce non-specific binding. The direct injection of a real sample with no pretreatment enabled us to complete the entire immunoassay in several minutes. This ease of operation and short measuring time are acceptable for on-site agricultural, environmentaland medical testing.
Keywords: SPR; immunoassay; raw sample; on-site; passive pump; microfluidics
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MDPI and ACS Style

Horiuchi, T.; Miura, T.; Iwasaki, Y.; Seyama, M.; Inoue, S.; Takahashi, J.-I.; Haga, T.; Tamechika, E. Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples. Sensors 2012, 12, 7095-7108.

AMA Style

Horiuchi T, Miura T, Iwasaki Y, Seyama M, Inoue S, Takahashi J-I, Haga T, Tamechika E. Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples. Sensors. 2012; 12(6):7095-7108.

Chicago/Turabian Style

Horiuchi, Tsutomu; Miura, Toru; Iwasaki, Yuzuru; Seyama, Michiko; Inoue, Suzuyo; Takahashi, Jun-ichi; Haga, Tsuneyuki; Tamechika, Emi. 2012. "Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples." Sensors 12, no. 6: 7095-7108.

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