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Sensors 2012, 12(6), 7080-7094; doi:10.3390/s120607080
Article

Quartz Crystal Microbalance Aptasensor for Sensitive Detection of Mercury(II) Based on Signal Amplification with Gold Nanoparticles

1
 and 1,2,*
Received: 18 April 2012; in revised form: 14 May 2012 / Accepted: 23 May 2012 / Published: 29 May 2012
(This article belongs to the Section Biosensors)
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Abstract: We show that a short mercury-specific aptamer (MSA) along with gold nanoparticles (Au-NPs) can be used to determine Hg(II) ion by a combination of a QCM-based sensor and a flow system. The MSA binds specifically to Hg(II), and the Au-NPs can amplify the signal to enhance sensitivity. Specifically, the short thiolated MSAs are immobilized on the surface of the QCM as the capture probe, and the MSAs are linked to the Au-NPs as the linking probe. The two components can form a sandwich structure of the T-Hg(II)-T type in the presence of Hg(II) ions. This leads to change in the mass on the QCM and a change in the resonance frequency. Hg(II) can be determined with a detection limit of 0.24 ± 0.06 nM which is better by three orders of magnitude than previous methods. The sensor can be regenerated by disrupting the T-Hg(II)-T base pairs with a solution of cysteine.
Keywords: mercury ion; aptamer; gold nanoparticle; quartz crystal microbalance; flow system mercury ion; aptamer; gold nanoparticle; quartz crystal microbalance; flow system
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Dong, Z.-M.; Zhao, G.-C. Quartz Crystal Microbalance Aptasensor for Sensitive Detection of Mercury(II) Based on Signal Amplification with Gold Nanoparticles. Sensors 2012, 12, 7080-7094.

AMA Style

Dong Z-M, Zhao G-C. Quartz Crystal Microbalance Aptasensor for Sensitive Detection of Mercury(II) Based on Signal Amplification with Gold Nanoparticles. Sensors. 2012; 12(6):7080-7094.

Chicago/Turabian Style

Dong, Zong-Mu; Zhao, Guang-Chao. 2012. "Quartz Crystal Microbalance Aptasensor for Sensitive Detection of Mercury(II) Based on Signal Amplification with Gold Nanoparticles." Sensors 12, no. 6: 7080-7094.


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