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Sensors 2012, 12(5), 5461-5470; doi:10.3390/s120505461

Detection of Prostate Stem Cell Antigen Expression in Human Prostate Cancer Using Quantum-Dot-Based Technology

1
Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China
2
Department of Urology, Angers Teaching Hospital, 4, Rue Larrey, Angers 49000, France
*
Author to whom correspondence should be addressed.
Received: 16 March 2012 / Revised: 13 April 2012 / Accepted: 19 April 2012 / Published: 27 April 2012
(This article belongs to the Special Issue Optical Sensors in Medicine)
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Abstract

Quantum dots (QDs) are a new class of fluorescent labeling for biological and biomedical applications. In this study, we detected prostate stem cell antigen (PSCA) expression correlated with tumor grade and stage in human prostate cancer by QDs-based immunolabeling and conventional immunohistochemistry (IHC), and evaluated the sensitivity and stability of QDs-based immunolabeling in comparison with IHC. Our data revealed that increasing levels of PSCA expression accompanied advanced tumor grade (QDs labeling, r = 0.732, p < 0.001; IHC, r = 0.683, p < 0.001) and stage (QDs labeling, r = 0.514, p = 0.001; IHC, r = 0.432, p = 0.005), and the similar tendency was detected by the two methods. In addition, by comparison between the two methods, QDs labeling was consistent with IHC in detecting the expression of PSCA in human prostate tissue correlated with different pathological types (K = 0.845, p < 0.001). During the observation time, QDs exhibited superior stability. The intensity of QDs fluorescence remained stable for two weeks (p = 0.083) after conjugation to the PSCA protein, and nearly 93% of positive expression with their fluorescence still could be seen after four weeks. View Full-Text
Keywords: prostate stem cell antigen; prostate cancer; quantum dots; fluorescence prostate stem cell antigen; prostate cancer; quantum dots; fluorescence
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ruan, Y.; Yu, W.; Cheng, F.; Zhang, X.; Larré, S. Detection of Prostate Stem Cell Antigen Expression in Human Prostate Cancer Using Quantum-Dot-Based Technology. Sensors 2012, 12, 5461-5470.

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