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Sensors 2012, 12(5), 5461-5470; doi:10.3390/s120505461
Article

Detection of Prostate Stem Cell Antigen Expression in Human Prostate Cancer Using Quantum-Dot-Based Technology

1
, 1
, 1,* , 1
 and 2
Received: 16 March 2012; in revised form: 13 April 2012 / Accepted: 19 April 2012 / Published: 27 April 2012
(This article belongs to the Special Issue Optical Sensors in Medicine)
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Abstract: Quantum dots (QDs) are a new class of fluorescent labeling for biological and biomedical applications. In this study, we detected prostate stem cell antigen (PSCA) expression correlated with tumor grade and stage in human prostate cancer by QDs-based immunolabeling and conventional immunohistochemistry (IHC), and evaluated the sensitivity and stability of QDs-based immunolabeling in comparison with IHC. Our data revealed that increasing levels of PSCA expression accompanied advanced tumor grade (QDs labeling, r = 0.732, p < 0.001; IHC, r = 0.683, p < 0.001) and stage (QDs labeling, r = 0.514, p = 0.001; IHC, r = 0.432, p = 0.005), and the similar tendency was detected by the two methods. In addition, by comparison between the two methods, QDs labeling was consistent with IHC in detecting the expression of PSCA in human prostate tissue correlated with different pathological types (K = 0.845, p < 0.001). During the observation time, QDs exhibited superior stability. The intensity of QDs fluorescence remained stable for two weeks (p = 0.083) after conjugation to the PSCA protein, and nearly 93% of positive expression with their fluorescence still could be seen after four weeks.
Keywords: prostate stem cell antigen; prostate cancer; quantum dots; fluorescence prostate stem cell antigen; prostate cancer; quantum dots; fluorescence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ruan, Y.; Yu, W.; Cheng, F.; Zhang, X.; Larré, S. Detection of Prostate Stem Cell Antigen Expression in Human Prostate Cancer Using Quantum-Dot-Based Technology. Sensors 2012, 12, 5461-5470.

AMA Style

Ruan Y, Yu W, Cheng F, Zhang X, Larré S. Detection of Prostate Stem Cell Antigen Expression in Human Prostate Cancer Using Quantum-Dot-Based Technology. Sensors. 2012; 12(5):5461-5470.

Chicago/Turabian Style

Ruan, Yuan; Yu, Weimin; Cheng, Fan; Zhang, Xiaobin; Larré, Stéphane. 2012. "Detection of Prostate Stem Cell Antigen Expression in Human Prostate Cancer Using Quantum-Dot-Based Technology." Sensors 12, no. 5: 5461-5470.


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