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Sensors 2012, 12(11), 15119-15132; doi:10.3390/s121115119
Article

Immuno-MALDI-MS in Human Plasma and On-Chip Biomarker Characterizations at the Femtomole Level

1,†
, 2,†
, 2
, 2
 and 1,*
Received: 8 October 2012; in revised form: 18 October 2012 / Accepted: 2 November 2012 / Published: 6 November 2012
(This article belongs to the Special Issue Biochips)
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Abstract: Immuno-SPR-MS is the combination of immuno-sensors in biochip format with mass spectrometry. This association of instrumentation allows the detection and the quantification of proteins of interest by SPR and their molecular characterization by additional MS analysis. However, two major bottlenecks must be overcome for a wide diffusion of the SPR-MS analytical platform: (i) To warrant all the potentialities of MS, an enzymatic digestion step must be developed taking into account the spot formats on the biochip and (ii) the biological relevancy of such an analytical solution requires that biosensing must be performed in complex media. In this study, we developed a procedure for the detection and the characterization at ~1 µg/mL of the LAG3 protein spiked in human plasma. The analytical performances of this new method was established, particularly its specificity (S/N > 9) and sensitivity (100% of LAG3 identification with high significant mascot score >68 at the femtomole level). The collective and automated on-chip MALDI-MS imaging and analysis based on peptidic fragments opens numerous applications in the fields of proteomics and diagnosis.
Keywords: Surface Plasmon Resonance; mass spectrometry; immuno MALDI-MS; biomarker; proteomics Surface Plasmon Resonance; mass spectrometry; immuno MALDI-MS; biomarker; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Rouleau, A.; Osta, M.E.; Lucchi, G.; Ducoroy, P.; Boireau, W. Immuno-MALDI-MS in Human Plasma and On-Chip Biomarker Characterizations at the Femtomole Level. Sensors 2012, 12, 15119-15132.

AMA Style

Rouleau A, Osta ME, Lucchi G, Ducoroy P, Boireau W. Immuno-MALDI-MS in Human Plasma and On-Chip Biomarker Characterizations at the Femtomole Level. Sensors. 2012; 12(11):15119-15132.

Chicago/Turabian Style

Rouleau, Alain; Osta, Marven E.; Lucchi, Géraldine; Ducoroy, Patrick; Boireau, Wilfrid. 2012. "Immuno-MALDI-MS in Human Plasma and On-Chip Biomarker Characterizations at the Femtomole Level." Sensors 12, no. 11: 15119-15132.



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