Next Article in Journal
Detailed Study of Amplitude Nonlinearity in Piezoresistive Force Sensors
Previous Article in Journal
Use of a New Ziprasidone-Selective Electrode in Mixed Solvents and Its Application in the Analysis of Pharmaceuticals and Biological Fluids
Sensors 2011, 11(9), 8826-8835; doi:10.3390/s110908826
Article

A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization

1
,
1,* , 2
 and
3
1 Department of Mechanical and Energy Engineering, University of North Texas, 3940 N. Elm St, Denton, TX 76207, USA 2 Department of Nanomechanical Systems, Korea Institute of Machinery and Materials, 156 Gajungbukno, Yuseong-gu, Daejeon 305-343, Korea 3 Department of Mechanical Engineering, POSTECH, San 31 Hyoja-Dong, Nam-Gu, Pohang, Gyungbuk 790-784, Korea
* Author to whom correspondence should be addressed.
Received: 5 August 2011 / Revised: 7 September 2011 / Accepted: 7 September 2011 / Published: 13 September 2011
(This article belongs to the Section Physical Sensors)
View Full-Text   |   Download PDF [593 KB, uploaded 21 June 2014]   |  

Abstract

We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of ~2 µm with an accuracy of ±0.01 °C. We produced and tested various micrometer-sized sensors, ranging from 2 µm to 30 µm. The sensor comprises unleaded low-melting-point solder alloy (Sn-based) as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating outside, creating a thermocouple junction at the tip. The sensor was calibrated using a thermally insulated calibration chamber, the temperature of which can be controlled with an accuracy of ±0.01 °C, and the thermoelectric power (Seebeck coefficient) of the sensor was recorded from 8.46 to 8.86 µV/°C. We have demonstrated the capability of measuring temperatures at a cellular level by inserting our temperature sensor into the membrane of a live retinal pigment epithelium cell subjected to a laser beam with a focal spot of 6 μm. We measured transient temperature profiles and the maximum temperatures were in the range of 38–55 ± 0.5 °C.
Keywords: micropipette; thermal sensor; cellular-level; laser micropipette; thermal sensor; cellular-level; laser
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Shrestha, R.; Choi, T.-Y.; Chang, W.; Kim, D. A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization. Sensors 2011, 11, 8826-8835.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert