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A Surface Plasmon Resonance Sensor for the Detection of Deoxynivalenol Using a Molecularly Imprinted Polymer
Food Safety Research Center, Korea Food Research Institute, Sungnam 463-746, South Korea
* Author to whom correspondence should be addressed.
Received: 15 July 2011; in revised form: 30 August 2011 / Accepted: 30 August 2011 / Published: 5 September 2011
Abstract: The aim of the present work was to investigate the feasibility of applying the molecular imprinting polymer technique to the detection of the mycotoxin deoxynivalenol (DON) using a surface plasmon resonance (SPR) transducer. A molecularly imprinted polypyrrole (MIPPy) film was prepared via electropolymerization of pyrrole onto a bare Au chip in the presence of a template DON molecule. Atomic force microscope SPR analysis showed that the MIPPy film was deposited homogeneously on the Au surface, with a thickness of 5 nm. The MIPPy–SPR sensor exhibited a linear response for the detection of DON in the range of 0.1–100 ng/mL (R2 = 0.988). The selectivity efficiency of the MIPPy film for DON and its acetylated analogs 3-ADON and 15-ADON was 100, 19, and 44%, respectively. The limit of detection for DON with the MIPPy–SPR for a standard solution was estimated at >1 ng/mL. These results suggest that the combination of SPR sensing with a MIPPy film as a synthetic receptor can be used to detect DON.
Keywords: deoxynivalenol; polypyrrole; molecular imprinting polymer; surface plasmon resonance; synthetic receptor
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MDPI and ACS Style
Choi, S.-W.; Chang, H.-J.; Lee, N.; Chun, H.S. A Surface Plasmon Resonance Sensor for the Detection of Deoxynivalenol Using a Molecularly Imprinted Polymer. Sensors 2011, 11, 8654-8664.
Choi S-W, Chang H-J, Lee N, Chun HS. A Surface Plasmon Resonance Sensor for the Detection of Deoxynivalenol Using a Molecularly Imprinted Polymer. Sensors. 2011; 11(9):8654-8664.
Choi, Sung-Wook; Chang, Hyun-Joo; Lee, Nari; Chun, Hyang Sook. 2011. "A Surface Plasmon Resonance Sensor for the Detection of Deoxynivalenol Using a Molecularly Imprinted Polymer." Sensors 11, no. 9: 8654-8664.