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Sensors 2011, 11(8), 8152-8163; doi:10.3390/s110808152

An Amperometric Biosensor for Glucose Determination Prepared from Glucose Oxidase Immobilized in Polyaniline-Polyvinylsulfonate Film

1
Department of Chemistry, Faculty of Sciences, Gazi University, Ankara 06500, Turkey
2
Department of Chemistry, Institute of Sciences, Gazi University, Ankara 06500, Turkey
*
Author to whom correspondence should be addressed.
Received: 24 June 2011 / Revised: 8 August 2011 / Accepted: 16 August 2011 / Published: 22 August 2011
(This article belongs to the Section Biosensors)
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Abstract

In this study, a novel amperometric glucose biosensor with immobilization of glucose oxidase on electrochemically polymerized polyaniline-polyvinylsulphonate (Pani-Pvs) films has been accomplished via the entrapment technique. Electropolymerization of aniline on the Pt surface of the Pt electrode was carried out at constant potential (0.75 V, vs. Ag/AgCl) using an electrochemical cell containing aniline and polyvinylsulphonate. Firstly, the optimum working conditions for preparing polyaniline-polyvinylsulfonate films were investigated. Determination of glucose was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and the optimum pH value was found to be 7.5. The storage stability and operational stability of the enzyme electrode were also studied. The results show that 75% of the response current was retained after 16 activity assays. The prepared glucose biosensor retained 80.6% of initial activity after 40 days when stored in 0.1 M phosphate buffer solution at 4 °C. View Full-Text
Keywords: glucose; biosensor; amperometry; polyaniline; polyvinylsulphonate glucose; biosensor; amperometry; polyaniline; polyvinylsulphonate
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MDPI and ACS Style

Arslan, F.; Ustabaş, S.; Arslan, H. An Amperometric Biosensor for Glucose Determination Prepared from Glucose Oxidase Immobilized in Polyaniline-Polyvinylsulfonate Film. Sensors 2011, 11, 8152-8163.

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