Sensors 2011, 11(5), 5520-5528; doi:10.3390/s110505520
Article

A Rapid Method to Regenerate Piezoelectric Microcantilever Sensors (PEMS)

LiNa Loo 1 email, Wei Wu 2 email, Wan Y. Shih 2 email, Wei-Heng Shih 2 email, Hossein Borghaei 1 email, Kambiz Pourrezaei 2 email and Gregory P. Adams 1,* email
1 Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave., Philadelphia, PA 19111, USA 2 Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA
* Author to whom correspondence should be addressed.
Received: 15 March 2011; in revised form: 10 May 2011 / Accepted: 17 May 2011 / Published: 20 May 2011
(This article belongs to the Special Issue Microcantilever Sensors)
PDF Full-text Download PDF Full-Text [232 KB, uploaded 20 May 2011 14:27 CEST]
Abstract: Piezoelectric microcantilever sensors (PEMS) can be sensitive tools for the detection of proteins and cells in biological fluids. However, currently available PEMS can only be used a single time or must be completely stripped and refunctionalized prior to subsequent uses. Here we report the successful use of an alternative regeneration protocol employing high salt concentrations to remove the target, leaving the functional probe immobilized on the microcantilever surface. Our model system employed the extracellular domain (ECD) of recombinant human Epidermal Growth Factor Receptor (EGFR) as the probe and anti-human EGFR polyclonal antibodies as the target. We report that high concentrations of MgCl2 dissociated polyclonal antibodies specifically bound to EGFR ECD immobilized on the sensor surface without affecting its bioactivity. This simple regeneration protocol both minimized the time required to re-conjugate the probe and preserved the density of probe immobilized on PEMS surface, yielding identical biosensor sensitivity over a series of assays.
Keywords: piezoelectric microcantilever; biosensor; regeneration; antibody; antigen; biomarker

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Loo, L.; Wu, W.; Shih, W.Y.; Shih, W.-H.; Borghaei, H.; Pourrezaei, K.; Adams, G.P. A Rapid Method to Regenerate Piezoelectric Microcantilever Sensors (PEMS). Sensors 2011, 11, 5520-5528.

AMA Style

Loo L, Wu W, Shih WY, Shih W-H, Borghaei H, Pourrezaei K, Adams GP. A Rapid Method to Regenerate Piezoelectric Microcantilever Sensors (PEMS). Sensors. 2011; 11(5):5520-5528.

Chicago/Turabian Style

Loo, LiNa; Wu, Wei; Shih, Wan Y.; Shih, Wei-Heng; Borghaei, Hossein; Pourrezaei, Kambiz; Adams, Gregory P. 2011. "A Rapid Method to Regenerate Piezoelectric Microcantilever Sensors (PEMS)." Sensors 11, no. 5: 5520-5528.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert