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System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells
Department of Mechanical Engineering, Southern Taiwan University, Tainan 71005, Taiwan
* Author to whom correspondence should be addressed.
Received: 8 October 2011; in revised form: 17 November 2011 / Accepted: 17 November 2011 / Published: 23 November 2011
(This article belongs to the Special Issue Biochips
Abstract: This paper develops a dielectrophoretic (DEP) chip with multi-layer electrodes and a micro-cavity array for programmable manipulations of cells and impedance measurement. The DEP chip consists of an ITO top electrode, flow chamber, middle electrode on an SU-8 surface, micro-cavity arrays of SU-8 and distributed electrodes at the bottom of the micro-cavity. Impedance sensing of single cells could be performed as follows: firstly, cells were trapped in a micro-cavity array by negative DEP force provided by top and middle electrodes; then, the impedance measurement for discrimination of different stage of bladder cancer cells was accomplished by the middle and bottom electrodes. After impedance sensing, the individual releasing of trapped cells was achieved by negative DEP force using the top and bottom electrodes in order to collect the identified cells once more. Both cell manipulations and impedance measurement had been integrated within a system controlled by a PC-based LabVIEW program. In the experiments, two different stages of bladder cancer cell lines (grade III: T24 and grade II: TSGH8301) were utilized for the demonstration of programmable manipulation and impedance sensing; as the results show, the lower-grade bladder cancer cells (TSGH8301) possess higher impedance than the higher-grade ones (T24). In general, the multi-step manipulations of cells can be easily programmed by controlling the electrical signal in our design, which provides an excellent platform technology for lab-on-a-chip (LOC) or a micro-total-analysis-system (Micro TAS).
Keywords: dielectrophoresis (DEP); single cell; manipulations; microstructure
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Chuang, C.-H.; Huang, Y.-W.; Wu, Y.-T. System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells. Sensors 2011, 11, 11021-11035.
Chuang C-H, Huang Y-W, Wu Y-T. System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells. Sensors. 2011; 11(11):11021-11035.
Chuang, Cheng-Hsin; Huang, Yao-Wei; Wu, Yao-Tung. 2011. "System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells." Sensors 11, no. 11: 11021-11035.