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Sensors 2011, 11(11), 10557-10570; doi:10.3390/s111110557

Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals

* ,
eBioscience, Inc., 10255 Science Center Drive, San Diego, CA 92121, USA
* Authors to whom correspondence should be addressed.
Received: 14 September 2011 / Revised: 26 October 2011 / Accepted: 26 October 2011 / Published: 7 November 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
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Advances in spectral deconvolution technologies are rapidly enabling researchers to replace or enhance traditional epifluorescence microscopes with instruments capable of detecting numerous markers simultaneously in a multiplexed fashion. While significantly expediting sample throughput and elucidating sample information, this technology is limited by the spectral width of common fluorescence reporters. Semiconductor nanocrystals (NC’s) are very bright, narrow band fluorescence emitters with great potential for multiplexed fluorescence detection, however the availability of NC’s with facile attachment chemistries to targeting molecules has been a severe limitation to the advancement of NC technology in applications such as immunocytochemistry and immunohistochemistry. Here we report the development of simple, yet novel attachment chemistries for antibodies onto NC’s and demonstrate how spectral deconvolution technology enables the multiplexed detection of 5 distinct NC-antibody conjugates with fluorescence emission wavelengths separated by as little as 20 nm.
Keywords: quantum dot; nanocrystal; IHC; immunohistochemistry; spectral imaging; multiplexing; conjugation; antibody quantum dot; nanocrystal; IHC; immunohistochemistry; spectral imaging; multiplexing; conjugation; antibody
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Jennings, T.L.; Triulzi, R.C.; Tao, G.; Louis, Z.E.S.; Becker-Catania, S.G. Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals. Sensors 2011, 11, 10557-10570.

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