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Sensors 2011, 11(11), 10388-10397; doi:10.3390/s111110388
Communication

Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates

, ,  and *
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
* Author to whom correspondence should be addressed.
Received: 26 September 2011 / Revised: 24 October 2011 / Accepted: 25 October 2011 / Published: 31 October 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
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Abstract

A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2. The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.
Keywords: sensor; glucose; chemiluminescence; DNAzyme; quantum dots; hydrogen peroxide; G-quadruplex; chemiluminescence resonance energy transfer sensor; glucose; chemiluminescence; DNAzyme; quantum dots; hydrogen peroxide; G-quadruplex; chemiluminescence resonance energy transfer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Niazov, A.; Freeman, R.; Girsh, J.; Willner, I. Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates. Sensors 2011, 11, 10388-10397.

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