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A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α
Beijing Institute of Pharmacology and Toxicology, No. 27, Taiping Road, Beijing 100850, China
Department of Pathology, General Hospital of the Chinese People’s Liberation Army, No. 28, Fuxing Road, Beijing 100853, China
These authors equally contributed to this work.
* Authors to whom correspondence should be addressed.
Received: 24 August 2011; in revised form: 11 October 2011 / Accepted: 1 November 2011 / Published: 3 November 2011
Abstract: We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs) fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α). In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor), on the surface of which the complementary sequences are linked (as cODN-AuNPs) and pre-hybridized with carboxymethylfluorescein (FAM)-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours.
Keywords: aptamers; gold nanoparticles; rHuEPO-α; fluorescent probe
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MDPI and ACS Style
Sun, J.; Guo, A.; Zhang, Z.; Guo, L.; Xie, J. A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α. Sensors 2011, 11, 10490-10501.
Sun J, Guo A, Zhang Z, Guo L, Xie J. A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α. Sensors. 2011; 11(11):10490-10501.
Sun, Jiefang; Guo, Aitao; Zhang, Zhaoyang; Guo, Lei; Xie, Jianwei. 2011. "A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α." Sensors 11, no. 11: 10490-10501.