Sensors 2011, 11(11), 10038-10047; doi:10.3390/s111110038
Article

Line-Monitoring, Hyperspectral Fluorescence Setup for Simultaneous Multi-Analyte Biosensing    

Received: 5 September 2011; in revised form: 5 October 2011 / Accepted: 17 October 2011 / Published: 25 October 2011
(This article belongs to the Special Issue Biochips)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Conventional fluorescence scanners utilize multiple filters to distinguish different fluorescent labels, and problems arise because of this filter-based mechanism. In this work we propose a line-monitoring, hyperspectral fluorescence technique which is designed and optimized for applications in multi-channel microfluidic systems. In contrast to the filter-based mechanism, which only records fluorescent intensities, the hyperspectral technique records the full spectrum for every point on the sample plane. Multivariate data exploitation is then applied to spectra analysis to determine ratios of different fluorescent labels and eliminate unwanted artifacts. This sensor is designed to monitor multiple fluidic channels simultaneously, providing the potential for multi-analyte biosensing. The detection sensitivity is approximately 0.81 fluors/μm2, and this sensor is proved to act with a good homogeneity. Finally, a model experiment of detecting short oligonucleotides has demonstrated the biomedical application of this hyperspectral fluorescence biosensor.
Keywords: hyperspectral fluorescence detection; microfluidic system; biosensor
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MDPI and ACS Style

Liu, Z.; Shi, H.; Liu, L.; Deng, S.; Ji, Y.; Ma, S.; Ma, H.; He, Y. Line-Monitoring, Hyperspectral Fluorescence Setup for Simultaneous Multi-Analyte Biosensing    . Sensors 2011, 11, 10038-10047.

AMA Style

Liu Z, Shi H, Liu L, Deng S, Ji Y, Ma S, Ma H, He Y. Line-Monitoring, Hyperspectral Fluorescence Setup for Simultaneous Multi-Analyte Biosensing    . Sensors. 2011; 11(11):10038-10047.

Chicago/Turabian Style

Liu, Zhiyi; Shi, Heng; Liu, Le; Deng, Sunan; Ji, Yanhong; Ma, Suihua; Ma, Hui; He, Yonghong. 2011. "Line-Monitoring, Hyperspectral Fluorescence Setup for Simultaneous Multi-Analyte Biosensing    ." Sensors 11, no. 11: 10038-10047.

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