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Sensors 2011, 11(8), 8018-8027; doi:10.3390/s110808018
Article

Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface

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Received: 16 June 2011 / Revised: 16 July 2011 / Accepted: 21 July 2011 / Published: 15 August 2011
(This article belongs to the Special Issue Biochips)
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Abstract

We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.
Keywords: electrochemical biosensor; single-nucleotide polymorphism (SNP); nonfouling electrode surface; Escherichia coli electrochemical biosensor; single-nucleotide polymorphism (SNP); nonfouling electrode surface; Escherichia coli
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Liu, G.; Lao, R.; Xu, L.; Xu, Q.; Li, L.; Zhang, M.; Shen, H.; Mathur, S.; Fan, C.; Song, S. Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface. Sensors 2011, 11, 8018-8027.

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