Sensors 2011, 11(8), 8018-8027; doi:10.3390/s110808018
Article

Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface

Gang Liu 1,2,* email, Ruojun Lao 2 email, Li Xu 1 email, Qin Xu 1 email, Lanying Li 1 email, Min Zhang 1 email, Hao Shen 3 email, Sanjay Mathur 3 email, Chunhai Fan 2 email and Shiping Song 2,* email
1 Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China 2 Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China 3 Institute of Inorganic Chemistry, University of Cologne, Cologne 50939, Germany
* Authors to whom correspondence should be addressed.
Received: 16 June 2011; in revised form: 16 July 2011 / Accepted: 21 July 2011 / Published: 15 August 2011
(This article belongs to the Special Issue Biochips)
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Abstract: We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.
Keywords: electrochemical biosensor; single-nucleotide polymorphism (SNP); nonfouling electrode surface; Escherichia coli

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Cite This Article

MDPI and ACS Style

Liu, G.; Lao, R.; Xu, L.; Xu, Q.; Li, L.; Zhang, M.; Shen, H.; Mathur, S.; Fan, C.; Song, S. Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface. Sensors 2011, 11, 8018-8027.

AMA Style

Liu G, Lao R, Xu L, Xu Q, Li L, Zhang M, Shen H, Mathur S, Fan C, Song S. Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface. Sensors. 2011; 11(8):8018-8027.

Chicago/Turabian Style

Liu, Gang; Lao, Ruojun; Xu, Li; Xu, Qin; Li, Lanying; Zhang, Min; Shen, Hao; Mathur, Sanjay; Fan, Chunhai; Song, Shiping. 2011. "Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface." Sensors 11, no. 8: 8018-8027.

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