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Sensors 2010, 10(6), 5927-5948; doi:10.3390/s100605927
Review

Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules

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Laboratoire de Physique Statistique de l’Ecole Normale Supérieure, associe aux Universites Paris 6 et Paris 7, CNRS UMR 8550, 24 rue Lhomond, 75005 Paris, France
* Author to whom correspondence should be addressed.
Received: 20 April 2010 / Revised: 10 May 2010 / Accepted: 28 May 2010 / Published: 10 June 2010
(This article belongs to the Special Issue Laser Spectroscopy and Sensing)
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Abstract

This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we discuss the lateral diffusion of membrane proteins, as measured by FRAP. Then, we talk about the use of FRAP to probe interactions between membrane proteins by obtaining fundamental information such as geometry and stoichiometry of the interacting complex. Afterwards, we discuss some applications of FRAP at the cellular level as well as the level of organisms. We conclude by comparing diffusion coefficients obtained by FRAP and several other alternative methods.
Keywords: biomembranes; Fluorescence Recovery after Photobleaching (FRAP); diffusion; biomolecular interactions biomembranes; Fluorescence Recovery after Photobleaching (FRAP); diffusion; biomolecular interactions
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Rayan, G.; Guet, J.-E.; Taulier, N.; Pincet, F.; Urbach, W. Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules. Sensors 2010, 10, 5927-5948.

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