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Sensors 2010, 10(6), 5758-5773; doi:10.3390/s100605758
Article

Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor

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Received: 26 April 2010 / Revised: 15 May 2010 / Accepted: 28 May 2010 / Published: 8 June 2010
(This article belongs to the Section Biosensors)
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Abstract

A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 µM in phosphate buffer and bovine serum media.
Keywords: diglyceride; disposable biosensor; enzyme diglyceride; disposable biosensor; enzyme
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hsu, S.-Y.; Bartling, B.; Wang, C.; Shieu, F.-S.; Liu, C.-C. Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor. Sensors 2010, 10, 5758-5773.

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