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Sensors 2010, 10(4), 2851-2868; doi:10.3390/s100402851

Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin

NanoScience and Sensor Technology Research Group, School of Applied Sciences and Engineering, Monash University, Churchill, Vic 3842, Australia
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Received: 2 January 2010 / Revised: 16 March 2010 / Accepted: 24 March 2010 / Published: 29 March 2010
(This article belongs to the Section Biosensors)
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Abstract

A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3,and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3–283 µM for penicillin and achieved a minimum detectable concentration of 0.3 µM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ± 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm. View Full-Text
Keywords: penicillin; penicillinase; polytyramine; biosensor; potentiometry penicillin; penicillinase; polytyramine; biosensor; potentiometry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ismail, F.; Adeloju, S.B. Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin. Sensors 2010, 10, 2851-2868.

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