Sensors 2010, 10(4), 2626-2637; doi:10.3390/s100402626
Article

Following Enzyme Activity with Infrared Spectroscopy

Saroj Kumaremail and Andreas Barth* email
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
* Author to whom correspondence should be addressed.
Received: 24 January 2010; in revised form: 20 February 2010 / Accepted: 6 March 2010 / Published: 25 March 2010
(This article belongs to the Special Issue Laser Spectroscopy and Sensing)
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Abstract: Fourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e.,no activity assay is required to convert the progress of the reaction into an observable quantity.
Keywords: vibrational spectroscopy; infrared spectroscopy; ATR; FTIR; enzyme activity

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MDPI and ACS Style

Kumar, S.; Barth, A. Following Enzyme Activity with Infrared Spectroscopy. Sensors 2010, 10, 2626-2637.

AMA Style

Kumar S, Barth A. Following Enzyme Activity with Infrared Spectroscopy. Sensors. 2010; 10(4):2626-2637.

Chicago/Turabian Style

Kumar, Saroj; Barth, Andreas. 2010. "Following Enzyme Activity with Infrared Spectroscopy." Sensors 10, no. 4: 2626-2637.

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