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Sensors 2010, 10(4), 2626-2637; doi:10.3390/s100402626
Article

Following Enzyme Activity with Infrared Spectroscopy

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Received: 24 January 2010 / Revised: 20 February 2010 / Accepted: 6 March 2010 / Published: 25 March 2010
(This article belongs to the Special Issue Laser Spectroscopy and Sensing)
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Abstract

Fourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e.,no activity assay is required to convert the progress of the reaction into an observable quantity.
Keywords: vibrational spectroscopy; infrared spectroscopy; ATR; FTIR; enzyme activity vibrational spectroscopy; infrared spectroscopy; ATR; FTIR; enzyme activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kumar, S.; Barth, A. Following Enzyme Activity with Infrared Spectroscopy. Sensors 2010, 10, 2626-2637.

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