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Sensors 2010, 10(3), 1846-1858; doi:10.3390/s100301846
Article

Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors

1
, 2
, 3
 and 1,*
1 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand 2 Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand 3 Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Prannok, Bangkok, 10700, Thailand
* Author to whom correspondence should be addressed.
Received: 25 January 2010 / Revised: 11 February 2010 / Accepted: 23 February 2010 / Published: 9 March 2010
(This article belongs to the Special Issue Microbial Sensors and Biosensors)
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Abstract

Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.
Keywords: Mycobacterium tuberculosis; piezoelectric biosensor; non-amplified genomic DNA Mycobacterium tuberculosis; piezoelectric biosensor; non-amplified genomic DNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kaewphinit, T.; Santiwatanakul, S.; Promptmas, C.; Chansiri, K. Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors. Sensors 2010, 10, 1846-1858.

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