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Design Strategies of Fluorescent Biosensors Based on Biological Macromolecular Receptors
Sensors 2010, 10(2), 1377-1398; doi:10.3390/s100201377

Whole-Cell Fluorescent Biosensors for Bioavailability and Biodegradation of Polychlorinated Biphenyls

Department of Science and Health, Institute of Technology Carlow, Kilkenny Road, Carlow, Ireland
* Author to whom correspondence should be addressed.
Received: 29 December 2009 / Revised: 14 January 2010 / Accepted: 29 January 2010 / Published: 21 February 2010
(This article belongs to the Special Issue Fluorescent Chemosensors)
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Whole-cell microbial biosensors are one of the newest molecular tools used in environmental monitoring. Such biosensors are constructed through fusing a reporter gene such as lux, gfp or lacZ,to a responsive promoter. There have been many reports of the applications of biosensors, particularly their use in assaying pollutant toxicity and bioavailability. This paper reviews the basic concepts behind the construction of whole-cell microbial biosensors for pollutant monitoring, and describes the applications of two such biosensors for detecting the bioavailability and biodegradation of Polychlorinated Biphenyls (PCBs).
Keywords: biosensor; Pseudomonas F113; PCB; biodegradation biosensor; Pseudomonas F113; PCB; biodegradation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Liu, X.; Germaine, K.J.; Ryan, D.; Dowling, D.N. Whole-Cell Fluorescent Biosensors for Bioavailability and Biodegradation of Polychlorinated Biphenyls. Sensors 2010, 10, 1377-1398.

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