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Reduced Fluoresceinamine as a Fluorescent Sensor for Nitric Oxide
REQUIMTE, Instituto Superior de Engenharia do Porto, R. António Bernardino Almeida 431, 4200-072 Porto, Portugal
Centro de Investigação em Química, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua Campo Alegre 687, 4169-007 Porto, Portugal
* Author to whom correspondence should be addressed.
Received: 28 December 2009; in revised form: 9 February 2010 / Accepted: 22 February 2010 / Published: 2 March 2010
Abstract: A new fluorescent sensor for nitric oxide (NO) is presented that is based on its reaction with a non fluorescent substance, reduced fluoresceinamine, producing the highly fluorescent fluoresceinamine. Using a portable homemade stabilized light source consisting of 450 nm LED and fiber optics to guide the light, the sensor responds linearly within seconds in the NO concentration range between about 10–750 μM with a limit of detection (LOD) of about 1 μM. The system generated precise intensity readings, with a relative standard deviation of less than 1%. The suitability of the sensor was assessed by monitoring the NO generated by either the nitrous acid decomposition reaction or from a NO-releasing compound. Using relatively high incubation times, the sensor also responds quantitatively to hydrogen peroxide and potassium superoxide, however, using transient signal measurements results in no interfering species.
Keywords: reduced fluoresceinamine; NO sensor; fluorescence; fiber optics
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Cite This Article
MDPI and ACS Style
Duarte, A.J.; Esteves da Silva, J.C. Reduced Fluoresceinamine as a Fluorescent Sensor for Nitric Oxide. Sensors 2010, 10, 1661-1669.
Duarte AJ, Esteves da Silva JC. Reduced Fluoresceinamine as a Fluorescent Sensor for Nitric Oxide. Sensors. 2010; 10(3):1661-1669.
Duarte, Abel J.; Esteves da Silva, Joaquim C.G. 2010. "Reduced Fluoresceinamine as a Fluorescent Sensor for Nitric Oxide." Sensors 10, no. 3: 1661-1669.