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Design Strategies of Fluorescent Biosensors Based on Biological Macromolecular Receptors
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan
CREST, JST, Uji, Kyoto 611-0011, Japan
* Author to whom correspondence should be addressed.
Received: 5 January 2010; in revised form: 29 January 2010 / Accepted: 4 February 2010 / Published: 12 February 2010
Abstract: Fluorescent biosensors to detect the bona fide events of biologically important molecules in living cells are increasingly demanded in the field of molecular cell biology. Recent advances in the development of fluorescent biosensors have made an outstanding contribution to elucidating not only the roles of individual biomolecules, but also the dynamic intracellular relationships between these molecules. However, rational design strategies of fluorescent biosensors are not as mature as they look. An insatiable request for the establishment of a more universal and versatile strategy continues to provide an attractive alternative, so-called modular strategy, which permits facile preparation of biosensors with tailored characteristics by a simple combination of a receptor and a signal transducer. This review describes an overview of the progress in design strategies of fluorescent biosensors, such as auto-fluorescent protein-based biosensors, protein-based biosensors covalently modified with synthetic fluorophores, and signaling aptamers, and highlights the insight into how a given receptor is converted to a fluorescent biosensor. Furthermore, we will demonstrate a significance of the modular strategy for the sensor design.
Keywords: design strategy of fluorescent biosensors; biological macromolecular receptor; genetically encoded fluorescent biosensors; chemically modified protein based sensors; signaling aptamers
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Tainaka, K.; Sakaguchi, R.; Hayashi, H.; Nakano, S.; Liew, F.F.; Morii, T. Design Strategies of Fluorescent Biosensors Based on Biological Macromolecular Receptors. Sensors 2010, 10, 1355-1376.
Tainaka K, Sakaguchi R, Hayashi H, Nakano S, Liew FF, Morii T. Design Strategies of Fluorescent Biosensors Based on Biological Macromolecular Receptors. Sensors. 2010; 10(2):1355-1376.
Tainaka, Kazuki; Sakaguchi, Reiko; Hayashi, Hironori; Nakano, Shun; Liew, Fong Fong; Morii, Takashi. 2010. "Design Strategies of Fluorescent Biosensors Based on Biological Macromolecular Receptors." Sensors 10, no. 2: 1355-1376.