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Nitrite Biosensing via Selective Enzymes—A Long but Promising Route
REQUIMTE—Departmento de Química, Faculdade de Ciencias e Tecnologia (UNL), 2829-516 Monte Caparica, Portugal
Escola Superior de Saude Egas Moniz, Campus Universitario, Quinta da Granja, 2829-511 Monte Caparica, Portugal
* Author to whom correspondence should be addressed.
Received: 9 October 2010; in revised form: 19 November 2010 / Accepted: 6 December 2010 / Published: 15 December 2010
Abstract: The last decades have witnessed a steady increase of the social and political awareness for the need of monitoring and controlling environmental and industrial processes. In the case of nitrite ion, due to its potential toxicity for human health, the European Union has recently implemented a number of rules to restrict its level in drinking waters and food products. Although several analytical protocols have been proposed for nitrite quantification, none of them enable a reliable and quick analysis of complex samples. An alternative approach relies on the construction of biosensing devices using stable enzymes, with both high activity and specificity for nitrite. In this paper we review the current state-of-the-art in the field of electrochemical and optical biosensors using nitrite reducing enzymes as biorecognition elements and discuss the opportunities and challenges in this emerging market.
Keywords: nitrite, biosensors; nitrite reductases; electrochemical transducers
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MDPI and ACS Style
Almeida, M.G.; Serra, A.; Silveira, C.M.; Moura, J.J. Nitrite Biosensing via Selective Enzymes—A Long but Promising Route. Sensors 2010, 10, 11530-11555.
Almeida MG, Serra A, Silveira CM, Moura JJ. Nitrite Biosensing via Selective Enzymes—A Long but Promising Route. Sensors. 2010; 10(12):11530-11555.
Almeida, M. Gabriela; Serra, Alexandra; Silveira, Celia M.; Moura, Jose J.G. 2010. "Nitrite Biosensing via Selective Enzymes—A Long but Promising Route." Sensors 10, no. 12: 11530-11555.