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Sensors 2010, 10(11), 10298-10313; doi:10.3390/s101110298

Biocatalysts Immobilized in Ultrathin Ordered Films

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Received: 29 September 2010; in revised form: 2 November 2010 / Accepted: 10 November 2010 / Published: 16 November 2010
(This article belongs to the Special Issue Bio-devices and Materials)
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Abstract: The immobilization of enzymes and other proteins into ordered thin materials has attracted considerable attention over the past few years. This research has demonstrated that biomolecules immobilized in different [Langmuir-Blodgett (LB)/Langmuir-Schaefer (LS)] matrixes retain their functional characteristics to a large extent. These new materials are of interest for applications as biosensors and biocatalysts. We review the growing field of oxidases immobilized onto ordered Langmiur-Blodgett and Langmuir-Schaefer films. Strategies for the preparation of solid supports and the essential properties of the resulting materials with respect to the envisaged applications are presented. Basic effects of the nature of the adsorption and various aspects of the application of these materials as biosensors, biocatalysts are discussed. Outlook of potential applications and further challenges are also provided.
Keywords: biosensors; enzyme immobilization; Langmuir-Blodgett technique; enzyme activity; AFM biosensors; enzyme immobilization; Langmuir-Blodgett technique; enzyme activity; AFM
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sołoducho, J.; Cabaj, J. Biocatalysts Immobilized in Ultrathin Ordered Films. Sensors 2010, 10, 10298-10313.

AMA Style

Sołoducho J, Cabaj J. Biocatalysts Immobilized in Ultrathin Ordered Films. Sensors. 2010; 10(11):10298-10313.

Chicago/Turabian Style

Sołoducho, Jadwiga; Cabaj, Joanna. 2010. "Biocatalysts Immobilized in Ultrathin Ordered Films." Sensors 10, no. 11: 10298-10313.

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