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Sensors 2010, 10(11), 10298-10313; doi:10.3390/s101110298

Biocatalysts Immobilized in Ultrathin Ordered Films

Department of Medicinal Chemistry and Microbiology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
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Received: 29 September 2010 / Revised: 2 November 2010 / Accepted: 10 November 2010 / Published: 16 November 2010
(This article belongs to the Special Issue Bio-devices and Materials)
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Abstract

The immobilization of enzymes and other proteins into ordered thin materials has attracted considerable attention over the past few years. This research has demonstrated that biomolecules immobilized in different [Langmuir-Blodgett (LB)/Langmuir-Schaefer (LS)] matrixes retain their functional characteristics to a large extent. These new materials are of interest for applications as biosensors and biocatalysts. We review the growing field of oxidases immobilized onto ordered Langmiur-Blodgett and Langmuir-Schaefer films. Strategies for the preparation of solid supports and the essential properties of the resulting materials with respect to the envisaged applications are presented. Basic effects of the nature of the adsorption and various aspects of the application of these materials as biosensors, biocatalysts are discussed. Outlook of potential applications and further challenges are also provided. View Full-Text
Keywords: biosensors; enzyme immobilization; Langmuir-Blodgett technique; enzyme activity; AFM biosensors; enzyme immobilization; Langmuir-Blodgett technique; enzyme activity; AFM
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Sołoducho, J.; Cabaj, J. Biocatalysts Immobilized in Ultrathin Ordered Films. Sensors 2010, 10, 10298-10313.

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