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Biocatalysts Immobilized in Ultrathin Ordered Films
Department of Medicinal Chemistry and Microbiology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
* Author to whom correspondence should be addressed.
Received: 29 September 2010; in revised form: 2 November 2010 / Accepted: 10 November 2010 / Published: 16 November 2010
Abstract: The immobilization of enzymes and other proteins into ordered thin materials has attracted considerable attention over the past few years. This research has demonstrated that biomolecules immobilized in different [Langmuir-Blodgett (LB)/Langmuir-Schaefer (LS)] matrixes retain their functional characteristics to a large extent. These new materials are of interest for applications as biosensors and biocatalysts. We review the growing field of oxidases immobilized onto ordered Langmiur-Blodgett and Langmuir-Schaefer films. Strategies for the preparation of solid supports and the essential properties of the resulting materials with respect to the envisaged applications are presented. Basic effects of the nature of the adsorption and various aspects of the application of these materials as biosensors, biocatalysts are discussed. Outlook of potential applications and further challenges are also provided.
Keywords: biosensors; enzyme immobilization; Langmuir-Blodgett technique; enzyme activity; AFM
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Sołoducho, J.; Cabaj, J. Biocatalysts Immobilized in Ultrathin Ordered Films. Sensors 2010, 10, 10298-10313.
Sołoducho J, Cabaj J. Biocatalysts Immobilized in Ultrathin Ordered Films. Sensors. 2010; 10(11):10298-10313.
Sołoducho, Jadwiga; Cabaj, Joanna. 2010. "Biocatalysts Immobilized in Ultrathin Ordered Films." Sensors 10, no. 11: 10298-10313.