Sensors 2010, 10(1), 697-718; doi:10.3390/s100100697
Article

Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System

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Received: 7 December 2009; in revised form: 8 January 2010 / Accepted: 12 January 2010 / Published: 19 January 2010
(This article belongs to the Special Issue DNA Sensors and Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.
Keywords: DNA quantification reliability; robust design; system identification model; real-time PCR machine; real-time PCR on-a-chip
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MDPI and ACS Style

Lee, D.-S. Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System. Sensors 2010, 10, 697-718.

AMA Style

Lee D-S. Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System. Sensors. 2010; 10(1):697-718.

Chicago/Turabian Style

Lee, Da-Sheng. 2010. "Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System." Sensors 10, no. 1: 697-718.

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