Sensors 2010, 10(1), 697-718; doi:10.3390/s100100697
Article

Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System

Department of Energy and Refrigerating Air Conditioning Engineering, National Taipei University of Technology, Taipei, Taiwan
Received: 7 December 2009; in revised form: 8 January 2010 / Accepted: 12 January 2010 / Published: 19 January 2010
(This article belongs to the Special Issue DNA Sensors and Biosensors)
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Abstract: Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.
Keywords: DNA quantification reliability; robust design; system identification model; real-time PCR machine; real-time PCR on-a-chip

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MDPI and ACS Style

Lee, D.-S. Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System. Sensors 2010, 10, 697-718.

AMA Style

Lee D-S. Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System. Sensors. 2010; 10(1):697-718.

Chicago/Turabian Style

Lee, Da-Sheng. 2010. "Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System." Sensors 10, no. 1: 697-718.

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