Sensors 2010, 10(1), 280-291; doi:10.3390/s100100280
Article

A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat

Dagmara Jacewicz1,* email, Aleksandra Dabrowska1 email, Dariusz Wyrzykowski1 email, Joanna Pranczk1 email, Michal Wozniak2 email, Jolanta Kubasik-Juraniec3 email, Narcyz Knap2 email, Kamila Siedlecka4 email, Alexander J. Neuwelt5 email and Lech Chmurzynski1 email
1 Department of General and Inorganic Chemistry, University of Gdańsk, Sobieskiego 18/19, 80-952 Gdańsk, Poland 2 Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland 3 Department of Electron Microscopy, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland 4 Department of Histology & Immunology, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland 5 Department of Neurology, Oregon Health & Sciences University, Portland OR 97239, USA
* Author to whom correspondence should be addressed.
Received: 4 November 2009; in revised form: 30 November 2009 / Accepted: 23 December 2009 / Published: 30 December 2009
(This article belongs to the Section Biosensors)
PDF Full-text Download PDF Full-Text [3091 KB, uploaded 30 December 2009 11:42 CET]
Abstract: The direct and accurate estimation of nitric dioxide levels is an extremely laborious and technically demanding procedure in the molecular diagnostics of inflammatory processes. The aim of this work is to demonstrate that a stop-flow technique utilizing a specific spectroscopic biosensor can be used for detection of nanomolar quantities of NO2 in biological milieu. The use of novel compound cis-[Cr(C2O4)(AaraNH2)(OH2)2]+ increases NO2 estimation accuracy by slowing down the rate of NO2 uptake. In this study, an animal model of pancreatitis, where nitrosative stress is induced by either 3g/kg bw or 1.5 g/kg bw dose of L-arginine, was used. Biochemical parameters and morphological characteristics of acute pancreatitis were monitored, specifically assessing pancreatic acinar cell death mode, NO2 generation and cellular glutathione level. The severity of the process correlated positively with NO2 levels in pancreatic acinar cell cytosol samples, and negatively with cellular glutathione levels.
Keywords: acute pancreatitis; nitrogen dioxide; L-arginine; Cr(III); rats; levels of inflammatory mediators; evaluation of apoptotic or necrotic effects

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Jacewicz, D.; Dabrowska, A.; Wyrzykowski, D.; Pranczk, J.; Wozniak, M.; Kubasik-Juraniec, J.; Knap, N.; Siedlecka, K.; Neuwelt, A.J.; Chmurzynski, L. A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat. Sensors 2010, 10, 280-291.

AMA Style

Jacewicz D., Dabrowska A., Wyrzykowski D., Pranczk J., Wozniak M., Kubasik-Juraniec J., Knap N., Siedlecka K., Neuwelt A.J., Chmurzynski L. A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat. Sensors. 2010; 10(1):280-291.

Chicago/Turabian Style

Jacewicz, Dagmara; Dabrowska, Aleksandra; Wyrzykowski, Dariusz; Pranczk, Joanna; Wozniak, Michal; Kubasik-Juraniec, Jolanta; Knap, Narcyz; Siedlecka, Kamila; Neuwelt, Alexander J.; Chmurzynski, Lech. 2010. "A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat." Sensors 10, no. 1: 280-291.

Sensors EISSN 1424-8220 Published by MDPI Publishing, Basel, Switzerland RSS E-Mail Table of Contents Alert