Sensors 2010, 10(1), 280-291; doi:10.3390/s100100280
Article

A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat

1 Department of General and Inorganic Chemistry, University of Gdańsk, Sobieskiego 18/19, 80-952 Gdańsk, Poland 2 Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland 3 Department of Electron Microscopy, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland 4 Department of Histology & Immunology, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland 5 Department of Neurology, Oregon Health & Sciences University, Portland OR 97239, USA
* Author to whom correspondence should be addressed.
Received: 4 November 2009; in revised form: 30 November 2009 / Accepted: 23 December 2009 / Published: 30 December 2009
(This article belongs to the Section Biosensors)
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Abstract: The direct and accurate estimation of nitric dioxide levels is an extremely laborious and technically demanding procedure in the molecular diagnostics of inflammatory processes. The aim of this work is to demonstrate that a stop-flow technique utilizing a specific spectroscopic biosensor can be used for detection of nanomolar quantities of NO2 in biological milieu. The use of novel compound cis-[Cr(C2O4)(AaraNH2)(OH2)2]+ increases NO2 estimation accuracy by slowing down the rate of NO2 uptake. In this study, an animal model of pancreatitis, where nitrosative stress is induced by either 3g/kg bw or 1.5 g/kg bw dose of L-arginine, was used. Biochemical parameters and morphological characteristics of acute pancreatitis were monitored, specifically assessing pancreatic acinar cell death mode, NO2 generation and cellular glutathione level. The severity of the process correlated positively with NO2 levels in pancreatic acinar cell cytosol samples, and negatively with cellular glutathione levels.
Keywords: acute pancreatitis; nitrogen dioxide; L-arginine; Cr(III); rats; levels of inflammatory mediators; evaluation of apoptotic or necrotic effects

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MDPI and ACS Style

Jacewicz, D.; Dabrowska, A.; Wyrzykowski, D.; Pranczk, J.; Wozniak, M.; Kubasik-Juraniec, J.; Knap, N.; Siedlecka, K.; Neuwelt, A.J.; Chmurzynski, L. A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat. Sensors 2010, 10, 280-291.

AMA Style

Jacewicz D, Dabrowska A, Wyrzykowski D, Pranczk J, Wozniak M, Kubasik-Juraniec J, Knap N, Siedlecka K, Neuwelt AJ, Chmurzynski L. A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat. Sensors. 2010; 10(1):280-291.

Chicago/Turabian Style

Jacewicz, Dagmara; Dabrowska, Aleksandra; Wyrzykowski, Dariusz; Pranczk, Joanna; Wozniak, Michal; Kubasik-Juraniec, Jolanta; Knap, Narcyz; Siedlecka, Kamila; Neuwelt, Alexander J.; Chmurzynski, Lech. 2010. "A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat." Sensors 10, no. 1: 280-291.

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