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Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
Institute of Chemistry, Federal University of Uberlândia, Uberlândia, 38400-902, Brazil
Institute of Genetics and Biochemistry, Federal Univ. of Uberlândia, Uberlândia, 38400-902, Brazil
* Author to whom correspondence should be addressed.
Received: 2 April 2008; in revised form: 11 June 2008 / Accepted: 12 June 2008 / Published: 8 July 2008
Abstract: This work describes the immobilization of purine and pyrimidine bases and immobilization/hybridization of synthetic oligonucleotides on graphite electrodes modified with poly(4-methoxyphenethylamine) produced in acid medium. The immobilization of adenine, guanine, cytosine and thymine on these modified electrodes was efficient, producing characteristic peaks. Another relevant observation is that, according to the literature, pyrimidine bases, cytosine and thymine are more difficult to detect. However, when immobilized onto the poly(4-methoxyphenethylamine), a significant increase in the magnitude of the current was obtained. The observation of the hybridization between the poly(GA) probe and its complementary, poly(CT) target, was possible by monitoring the guanosine and adenosine peaks or through methylene blue indicator, using differential pulse voltammetry. Hybridization results in a decrease of the peak current of guanosine and adenosine or the signal of methylene blue accumulated on the modified electrode surface. The hybridization with the complementary target was also investigated by electrochemical impedance spectroscopy. The results showed a significant modification in the Nyquist plot, after addition of the complementary target, with increase of the charge transference resistance.
Keywords: electrochemical investigation; hybridization; immobilization; nucleotides; oligonucleotides; voltammetry
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Silva, F.B.; Vieira, S.N.; Goulart, L.R., Filho; Boodts, J.F.C.; Brito-Madurro, A.G.; Madurro, J.M. Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine). Int. J. Mol. Sci. 2008, 9, 1173-1187.
Silva FB, Vieira SN, Goulart LR, Filho, Boodts JFC, Brito-Madurro AG, Madurro JM. Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine). International Journal of Molecular Sciences. 2008; 9(7):1173-1187.
Silva, Francielle B.; Vieira, Sabrina N.; Goulart, Luiz R., Filho; Boodts, Julien F.C.; Brito-Madurro, Ana G.; Madurro, João M. 2008. "Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)." Int. J. Mol. Sci. 9, no. 7: 1173-1187.