Next Article in Journal
Correlation of the Rates of Solvolysis of Two Arenesulfonyl Chlorides and of trans-β-Styrenesulfonyl Chloride — Precursors in the Development of New Pharmaceuticals
Next Article in Special Issue
Quantum Dots for Live Cell and In Vivo Imaging
Previous Article in Journal
Occurrence of Aflatoxin M1 in Dairy Products in Southern Italy
Previous Article in Special Issue
Preparation and Characterization of Highly Fluorescent, Glutathione-coated Near Infrared Quantum Dots for in Vivo Fluorescence Imaging
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2008, 9(12), 2622-2638; doi:10.3390/ijms9122622

Potential Use of Quantum Dots in Flow Cytometry

1
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia
2
Department of Molecular Medicine & Pathology, University of Auckland Medical School, Auckland, New Zealand
3
Department of Earth Sciences, Macquarie University, Sydney, Australia
4
Department of Physics, Macquarie University, Sydney, Australia
5
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
*
Author to whom correspondence should be addressed.
Received: 4 July 2008 / Revised: 20 November 2008 / Accepted: 10 December 2008 / Published: 17 December 2008
View Full-Text   |   Download PDF [458 KB, uploaded 19 June 2014]   |  

Abstract

QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC. View Full-Text
Keywords: Quantum dots; flow cytometry; fluorescent detection; detection limit; autofluorescence Quantum dots; flow cytometry; fluorescent detection; detection limit; autofluorescence
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ibáñez-Peral, R.; Bergquist, P.L.; Walter, M.R.; Gibbs, M.; Goldys, E.M.; Ferrari, B. Potential Use of Quantum Dots in Flow Cytometry. Int. J. Mol. Sci. 2008, 9, 2622-2638.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top