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Potential Use of Quantum Dots in Flow Cytometry
AbstractQDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
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Ibáñez-Peral, R.; Bergquist, P.L.; Walter, M.R.; Gibbs, M.; Goldys, E.M.; Ferrari, B. Potential Use of Quantum Dots in Flow Cytometry. Int. J. Mol. Sci. 2008, 9, 2622-2638.View more citation formats
Ibáñez-Peral R, Bergquist PL, Walter MR, Gibbs M, Goldys EM, Ferrari B. Potential Use of Quantum Dots in Flow Cytometry. International Journal of Molecular Sciences. 2008; 9(12):2622-2638.Chicago/Turabian Style
Ibáñez-Peral, Raquel; Bergquist, Peter L.; Walter, Malcolm R.; Gibbs, Moreland; Goldys, Ewa M.; Ferrari, Belinda. 2008. "Potential Use of Quantum Dots in Flow Cytometry." Int. J. Mol. Sci. 9, no. 12: 2622-2638.