Int. J. Mol. Sci. 2008, 9(12), 2622-2638; doi:10.3390/ijms9122622
Article

Potential Use of Quantum Dots in Flow Cytometry

1,* email, 1,2email, 3email, 1email, 4email and 1,5email
Received: 4 July 2008; in revised form: 20 November 2008 / Accepted: 10 December 2008 / Published: 17 December 2008
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
Keywords: Quantum dots; flow cytometry; fluorescent detection; detection limit; autofluorescence
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MDPI and ACS Style

Ibáñez-Peral, R.; Bergquist, P.L.; Walter, M.R.; Gibbs, M.; Goldys, E.M.; Ferrari, B. Potential Use of Quantum Dots in Flow Cytometry. Int. J. Mol. Sci. 2008, 9, 2622-2638.

AMA Style

Ibáñez-Peral R, Bergquist PL, Walter MR, Gibbs M, Goldys EM, Ferrari B. Potential Use of Quantum Dots in Flow Cytometry. International Journal of Molecular Sciences. 2008; 9(12):2622-2638.

Chicago/Turabian Style

Ibáñez-Peral, Raquel; Bergquist, Peter L.; Walter, Malcolm R.; Gibbs, Moreland; Goldys, Ewa M.; Ferrari, Belinda. 2008. "Potential Use of Quantum Dots in Flow Cytometry." Int. J. Mol. Sci. 9, no. 12: 2622-2638.

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