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Potential Use of Quantum Dots in Flow Cytometry
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia
Department of Molecular Medicine & Pathology, University of Auckland Medical School, Auckland, New Zealand
Department of Earth Sciences, Macquarie University, Sydney, Australia
Department of Physics, Macquarie University, Sydney, Australia
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
* Author to whom correspondence should be addressed.
Received: 4 July 2008; in revised form: 20 November 2008 / Accepted: 10 December 2008 / Published: 17 December 2008
Abstract: QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
Keywords: Quantum dots; flow cytometry; fluorescent detection; detection limit; autofluorescence
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Ibáñez-Peral, R.; Bergquist, P.L.; Walter, M.R.; Gibbs, M.; Goldys, E.M.; Ferrari, B. Potential Use of Quantum Dots in Flow Cytometry. Int. J. Mol. Sci. 2008, 9, 2622-2638.
Ibáñez-Peral R, Bergquist PL, Walter MR, Gibbs M, Goldys EM, Ferrari B. Potential Use of Quantum Dots in Flow Cytometry. International Journal of Molecular Sciences. 2008; 9(12):2622-2638.
Ibáñez-Peral, Raquel; Bergquist, Peter L.; Walter, Malcolm R.; Gibbs, Moreland; Goldys, Ewa M.; Ferrari, Belinda. 2008. "Potential Use of Quantum Dots in Flow Cytometry." Int. J. Mol. Sci. 9, no. 12: 2622-2638.