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Int. J. Mol. Sci. 2008, 9(12), 2622-2638; doi:10.3390/ijms9122622

Potential Use of Quantum Dots in Flow Cytometry

1,* , 1,2
1 Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia 2 Department of Molecular Medicine & Pathology, University of Auckland Medical School, Auckland, New Zealand 3 Department of Earth Sciences, Macquarie University, Sydney, Australia 4 Department of Physics, Macquarie University, Sydney, Australia 5 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
* Author to whom correspondence should be addressed.
Received: 4 July 2008 / Revised: 20 November 2008 / Accepted: 10 December 2008 / Published: 17 December 2008
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QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
Keywords: Quantum dots; flow cytometry; fluorescent detection; detection limit; autofluorescence Quantum dots; flow cytometry; fluorescent detection; detection limit; autofluorescence
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Ibáñez-Peral, R.; Bergquist, P.L.; Walter, M.R.; Gibbs, M.; Goldys, E.M.; Ferrari, B. Potential Use of Quantum Dots in Flow Cytometry. Int. J. Mol. Sci. 2008, 9, 2622-2638.

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